BLKIM051c Clinical Immunology - practice

Faculty of Medicine
Autumn 2011
Extent and Intensity
0/0/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (lecturer)
RNDr. Jana Nechvátalová, Ph.D. (seminar tutor)
Mgr. Olga Tichá, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: Miroslava Bučková
Prerequisites (in Czech)
BLKFY0222p Physiology II-lec.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The practical course is are focussed to methods used in laboratory diagnostics of immunopathological states, to indication and interpretation of laboratory immunological test and diagnostic algorithms of immunopathological diseases. After finishing the course the student will be able to apply and interpret basic immunological laboratory tests. The sudents must manage laboraty tests protocol preparation of the tests used.
Syllabus
  • Antigens, immunization, serum, antisera preparation.
  • Monoclonal antibodies.
  • Principles of reaction of antigen plus antiboidy.
  • Agglutination.
  • Precipitation in gel and solution. Immunoelectrophoresis, immunofixation.
  • Immunoassays, imunofluorescence, ELISA, RIA, LIA.
  • Isolation of the cells of the immune system from blod and other biological material.
  • Flow cytometry.
  • Lymphocyte cultivation in vivo, proliferation tests, cytokine production. Tests for in-vitro production of antibodies, ELISPOT.
  • Phagocytic tests.
  • Complement system evaluation.
  • Good laboratory practice.
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • BARTŮŇKOVÁ, Jiřina and Milan PAULÍK. Vyšetřovací metody v imunologii. Vyd. 1. Praha: Grada, 2005, 176 s. ISBN 8024706911. info
Teaching methods
Laboratory practice. Home preparation for the practice is required.
Assessment methods
To obtain the credit presence in 90% of the lectures is required. The quality of laboratory protocols is assessed.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 10.
The course is also listed under the following terms Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2011/BLKIM051c