LF:BLMB011c Mol.andCell.Biol.-pract.

BLMB011c Molecular and Cellular Biology - practice

Faculty of Medicine
autumn 2019

Extent and Intensity

0/3/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Guaranteed by

prof. Ing. Petr Dvořák, CSc.
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Ing. Lívia Eiselleová, Ph.D.
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine

Timetable

Wed 7:30–8:30 B07/205a, Wed 8:30–9:30 B07/217, Wed 8:30–9:30 B07/205a, Wed 9:30–10:00 B07/205a

Prerequisites

Knowledge of high-school biology on the level of Overview of High School Biology (https://is.muni.cz/auth/do/med/el/overview/overview_CZ.qwarp) or similar is required.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

Medical Laboratory Technologist (programme LF, B-LABD)
Laboratory Assistant (programme LF, B-SZ)

Course objectives

The main objectives of the course are:
- learning to operate the light microscope while observing prokaryotic and eukaryotic cells and various cell structures and processes
- understanding the methods of risk evaluation for monogenic hereditary diseases with autosomal inheritance in patients based on family anamneses

Learning outcomes

After completing the course, the student will be able to:
- operate the light microscope
- perform some of the basic cytochemical staining techniques in observation of bacterial and human cells
- explain the basic principles and use of electron microscopy
- describe the basic techniques and applications of tissue and cell cultures
- deduce the impact of selected DNA mutations on the primary structure of proteins
- explain the methodology of assembling and examining the karyotype and its use, and to evaluate basic numerical and structural aberrations in assigned human karyotypes
- evaluate the risk of numerical and structural aberrations resulting from meiotic non-disjunctions and chromosomal translocations
- evaluate the risk of monogenic hereditary diseases with autosomal inheritance in patients based on assigned family anamneses of the diseases

Syllabus

Week 1: Instructions. Non-cellular organisms and infectious agents
Week 2: Prokaryotic cells and their medical significance
Week 3: Microscopic observation of eukaryotic cells
Week 4: In vitro culture of human cells
Week 5: DNA structure and replication. Transcription and translation
Week 6: Principles of electron microscopy, cellular ultrastructure
Week 7: Plasma membrane - osmosis, cell fusion
Week 8: Methods of cell cycle studying
Week 9: Mitosis observation in a light microscope
Week 10: Biological significance of meiosis - gametogenesis
Week 11: Human karyotype and chromosomal abnormalities
Week 12: Genetic disorders - autosomal inheritance
Week 13: substitution week

Literature

required literature

Aktuální protokoly ke cvičením jsou dostupné v elektronické podobě na stránkách informačního systému MU (ISu): Studijní materiály předmětu BLMB011c

Teaching methods

laboratory practice, class discussion

Assessment methods

Practical classes are mandatory.
Requirements for the course completion: full attendance in the practices (1 absence, excused or unexcused, is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; activity in the practices.
In case of student’s late arrival to the practice or poor activity at the practice, the student is required to write an essay in the length of 2 pages on a given topic. Same for the case of 2nd and any additional absence in practices (excused or unexcused). Find the instructions for essay writing in the Course-related instructions in the IS, bring the essay printed to the teacher who specified the topic; be ready to answer the teacher's questions regarding the topic.
In case of 3 absences, (or more than 3 absences all excused by the Office for Studies within 5 days from the beginning of the absence and introduced to the Information System), it is addressed individually and course-unit credit is awarded only after the proof of the student's knowledge of the entire semester topics from lectures and practices in a Credit test (20 questions: 10 test questions, multiple answers can be correct, negative marking is obtained for incorrect answers + 10 given terms for written explanation. At least 10 points out of 20 are needed to pass.)
In the case of 4 or more unexcused absences, the course-unit credit is not awarded.

Language of instruction

Czech

Follow-Up Courses

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 45.

Listed among pre-requisites of other courses

Teacher's information

https://is.muni.cz/auth/el/1411/podzim2019/BLMB011c/index.qwarp
What to bring to each practice: printed protocol for the respective practice, your own writing materials. Download the protocol for each practice from the Study materials in the IS, and study it in advance. Lab shoes, lab coats and disposable gloves are not required, but for the tasks with staining, lab coats are recommended.

Complete Biology instructions to be found at the following link: https://is.muni.cz/auth/el/1411/podzim2018/BLMB011c/op/Pokyny_ke_cvicenim_ZDRL_denni.pdf

The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

Enrolment Statistics (autumn 2019, recent)
Permalink: https://is.muni.cz/course/med/autumn2019/BLMB011c

LF:BLMB011c Mol.andCell.Biol.-pract. - Course Information

BLMB011c Molecular and Cellular Biology - practice

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