LF:VLMB011 Imaging and analytical methods - Course Information

VLMB011 Modern imaging and analytical methods in cellular biology

Extent and Intensity

0/0/.6. 3 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

Mgr. Kamila Bendíčková, Ph.D. (lecturer)
Mgr. Jan Frič, Ph.D. (lecturer)
Mgr. Marcela Hortová Kohoutková, Ph.D. (lecturer)
Mgr. Petra Lázničková, Ph.D. (lecturer)
Mgr. Ivana Andrejčinová (seminar tutor)
Mgr. Veronika Bosáková (seminar tutor)
Monica Feole, M.Sc. (seminar tutor)
Mgr. Filip Kafka (seminar tutor)
Ioanna Papatheodorou (seminar tutor)
Mgr. Ondřej Vymazal (seminar tutor)

Guaranteed by

Mgr. Jan Frič, Ph.D.
Department of Biology – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine

Timetable

Fri 13. 10. 9:00–15:00 Virtuální místnost LF/Virtual room LF, Fri 27. 10. 9:00–15:00 A 1.08

Prerequisites

VLBI0222p Biology II-lect. || ZLBI0222p Biology II-lect. || PřF:Bi1700 Cell Biology || VSBI0222p Biology II-lect.
Students need to have cell biology knowledge at the level of 1st year of university, proven with an exam (VLBI0222p, ZLBI0222p or Bi1700), knowledge of Czech language.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 9/15, only registered: 0/15

fields of study / plans the course is directly associated with

• General Medicine (programme LF, M-VL) (2)
• Dentistry (programme LF, M-ZL) (2)

Course objectives

After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); confocal microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.

Learning outcomes

Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.

Syllabus

• *All lessons are taught in the Czech language*
• 1st Friday (13.10.2023): Online lectures 9:00-15:00 (MS Teams)
• Lessons will be recorded and uploaded into study materials:
• - Principles of confocal microscopy and applications
• - Principles of flow cytometry and cell phenotype analysis using flow cytometry
• - Cell separation methods (FACS sorting)
• - Advanced flow cytometry methods, Spectral cytometry, Mass cytometry
• - Advanced microscopy techniques - electron and multiphoton microscopy
• - Other molecular biology methods for proteins and RNA analysis
• Friday (27.10.): In-person meeting (Biology park Bohunice near Campus)
• Morning session 9:00-11.15
• - Lab tour with methods demonstration
• Afternoon session from 12:00
• - Imaging and analytical methods in clinical research
• Final test (14:00):
• - 2 open questions from each theoretical online presentation
• *All lessons are taught in the Czech language -for the English course check LF: aVLMB031
• Contact person: ovymazal@mail.muni.cz

Literature

recommended literature
• prezentace z přednášek

Teaching methods

seminar, lab demonstration

Assessment methods

Course completion requires attendance and passing a final written test (10 questions about the basic principles of demonstrated methods).
Attendance at an online seminar is highly recommended; in-person seminars require full attendance. Attendance at all seminars is mandatory

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 10.
Information on course enrolment limitations: Pokud nemá student zkoušku z Biologie II, musí mít absolvovaný předmět Bunečná biologie Bi1700 na PřF.

Teacher's information

https://is.muni.cz/auth/el/1411/podzim2022/VLMB011/index.qwarp
The teaching takes place in blocks on two Fridays (13.10. a 27.10.2023).

• Enrolment Statistics (recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2023/VLMB011