VSBC041c Biochemistry II -practice

Faculty of Medicine
Spring 2003
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Hana Bochořáková (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
doc. RNDr. Jaroslava Tallová, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Lenka Nerudová (assistant)
Guaranteed by
prof. MUDr. Vladimír Palyza, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Handling biological material. Anticoagulants and their use for blood plasma separation. Communicating results of tests in clinical biochemistry: request forms and laboratory reports.
  • Analysis of the absorption spectra of haemoglobin, oxyhaemoglobin and carbonylhaemoglobin. Determination of haemoglobin in blood. Urine test for haemoglobin.
  • Determination of total bilirubin in serum. Estimation of bilirubin, urobilinogens and urobilins in urine.
  • Determination of total serum proteins by biuret reagent, determination of albumin. Precipitation tests for urine proteins. Detection of proteinuria by diagnostic strips.
  • Determination of alanine aminotransferase activity in serum. Determination of alkaline phosphatase activity in serum.
  • The enzymatic assay of glucose in blood and urine. Oral glucose tolerance test. Estimation of glycaemia by diagnostic strips. Detection of glucose in urine.
  • Determination of total cholesterol in serum. Principles of HDL-cholesterol determination and calculation of the LDL-cholesterol concentration. Chromatography of serum lipids.
  • The solubility of uric acid. Murexide test for uric acid. Detection of uric acid in renal calculi. Determination of uric acid in serum and urine.
  • Determination of urea in serum and urine. Approximate assessment of the nitrogen balance.
  • Appearance and volume of urine, specific gravity and osmolality of urine. Urine pH measurement. Determination of creatinine in blood, serum and urine. Creatinine clearance calculation, calculation of tubular reabsorption and fractional excretion of water.
  • Tests in routine urinalysis employing chemical principle: determination of pathological components, multipurpose diagnostic strips in urinalysis.
  • Simplified chemical analysis of renal calculi. Practice in urinalysis, seeking for pathological constituents in urine.
  • Determination of gastric acid output. Tubeless gastric analyses. Detection of lactic acid.
  • Imunochemical assays.
Literature
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu
Listed among pre-requisites of other courses
Teacher's information
http://www.med.muni.cz/toCP1250.cs/biochem/vyuka.html
The course is also listed under the following terms Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016.
  • Enrolment Statistics (Spring 2003, recent)
  • Permalink: https://is.muni.cz/course/med/spring2003/VSBC041c