Information System of Masaryk University 

Course Information

ZLBC041c Biochemistry II -practice

Faculty of Medicine
Spring 2012

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

PharmDr. Lenka Adámková, Ph.D. (seminar tutor)
RNDr. Hana Bochořáková (seminar tutor)
Ing. Martina Čarnecká, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová (seminar tutor)
Mgr. Kateřina Chvátalová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
Mgr. Milena Matejovičová, Ph.D. (seminar tutor)
Ing. Jitka Melounová, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Ing. Jana Simonová, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová (seminar tutor)
Lenka Nerudová (assistant)

Supervisor

prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Dodavatelské pracoviště: Department of Biochemistry - Theoretical Departments - Faculty of Medicine

Timetable of Seminar Groups

ZLBC041c/21 each even Thursday 7:30–9:10 N02015
ZLBC041c/22 each even Wednesday 7:30–9:10 N02015
ZLBC041c/23 each odd Thursday 7:30–9:10 N02015
ZLBC041c/24 each odd Wednesday 7:30–9:10 N02015
ZLBC041c/40 J. Tomandl

Prerequisites (v češtině)

ZLLC011p Medical Chemistry && ZLBF011p Med.Physics and Informat.-lect && ZLBI0222p Biology II-lect. && ZLBC0221s Biochemistry I - seminar && ZLFY0321c Physiol. I - pract. && ZLFY0321s Physiol. I - sem.

Course Enrollment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

Fields of study the course is directly associated with

• Dentistry (programme LF, M-ZL)
• Dentistry (eng.) (programme LF, M-ZL)

Course objectives

Course is complementary to VSLC0421p and VSLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics

Syllabus

• Handling biological material. Drawing blood specimens. 1.2 Blood serum. Anticoagulants, blood plasma. 1.3 Biochemical analyzers. 1.4 Interpretation of test results. 1.5 Reporting of test results Laboratory investigations of lipid and cholesterol metabolism 2.1 The enzymatic assay of total serum cholesterol. 2.2 Determination of serum triacylglycerols. 2.3 HDL-cholesterol determination, calculation of the LDL-cholesterol. 2.4 The chylomicron test. 2.5 Electrophoresis of lipoproteins. Investigations of saccharide metabolism. Diabetes mellitus 3.1 The enzymatic assay of glucose in blood. 3.2 Estimation of blood glucose using a personal glucometer. 3.3 The oral glucose tolerance test (oGTT). 3.4 Detection and determination of glucose in urine. 3.5 Detection of ketone bodies in urine. 3.6 Determination of glycated haemoglobin (HbA1) in blood. 3.7 The long-term complications of diabetes – microalbuminuria. Selected investigations in liver diseases 4.1 Determination of alanine aminotransferase (ALT) activity. 4.2 Determination of aspartate aminotransferase (AST) activity. 5.2 Detection of bilirubin in urine. 5.3 Detection of urobilinogens in urine. Laboratory diagnostics of myocardial infarction 6.1 Measurement of total plasma creatine kinase activity. 6.2 Measurement of plasma CK-MB activity. 6.3 Measurement of plasma troponin T concentration. 6.4 Measurement of plasma myoglobin concentration. Investigations of plasma proteins 7.1 Determination of total serum proteins by the biuret method. 7.2 Determination of blood serum albumin. 7.3 Electrophoresis of serum proteins. 7.4 Assay for increased level of C-reactive protein. 7.5 Determination of IgE in blood serum (ELISA). 7.6 Determination of ferritin in blood serum (ELISA). Compensatory practicals. Credits.

Literature

• Biochemistry. Edited by Josef Tomandl - Eva Táborská. 1st ed. Brno: Masaryk University, 2008. 81 s. ISBN 978-80-210-4547-7. info
• Biochemie.Praktická cvičení. Edited by Eva Táborská - Josef Tomandl - Hana Paulová. 3. přeprac. vyd. Brno: Masarykova univerzita, 2008. 126 s. ISBN 978-80-210-4516-3. info

Teaching methods

laboratory practicals

Assessment methods

Practicals in biochemistry lab.
Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports and handing them to the teacher till the given data. Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry I.

Further comments (probably available only v češtině)

The course is taught annually.
Information on course enrollment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu

Listed among pre-requisites of other courses

• ZLCP051c Clinical Examination in Surgery - practice
  ZLFY0422c && ZLFY0422s && ZLBC041c && ZLBC041s && ZLAN0333p && ZLHE0322p && (ZLLT0222s || ZLLT0222p) && ZLET021p && ZLPO011p && ZC041  

The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2013, Spring 2014.

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• Enrollment Statistics (Spring 2012, recent)
  
• Permalink: https://is.muni.cz/course/med/spring2012/ZLBC041c

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