FaF:F1BL1_16 Biotechnology of Drugs - Course Information

F1BL1_16 Biotechnology of Drugs

Extent and Intensity

3/0/0. 3 credit(s). Type of Completion: zk (examination).
Taught online.

Teacher(s)

Mgr. Marie Brázdová, Ph.D. (lecturer)
prof. Ing. René Kizek, DrSc., MBA (lecturer)

Guaranteed by

prof. Ing. René Kizek, DrSc., MBA
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy

Timetable

Wed 7:30–9:45 44-056

Prerequisites (in Czech)

3120/F1ME1 Molekulární biologie
3120/F1MG1 Metody molekulární biologie

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 65 student(s).
Current registration and enrolment status: enrolled: 3/65, only registered: 0/65, only registered with preference (fields directly associated with the programme): 0/65

fields of study / plans the course is directly associated with

• Pharmacy (programme FaF, M-FARM)
• Multidisciplinary studies at Faculty of Pharmacy (programme CST, KOS)

Course objectives

This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.

Learning outcomes

Basic knowledge from cytology, genetic engineering, and industrial microbiology which are used in preparation of recombinant pharmaceuticals. Overview of biotechnology production of antibiotics, vitamins, organic acids, amino acids and alkaloids.

Syllabus

• 1) Introduction to pharmaceutical biotechnology, definition, milestones
  2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones
  3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors
  4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification
  5) Expression of recombinant proteins in prokaryotic cells and yeasts
  6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses
  7) The classical biotechnological processes in pharmacology
  8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification
  9) Examples of classical and recombinant biotechnology products in pharmacy, cytokines, hormones, enzymes, antibodies and their derivates
  10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes
  11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming
  12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry, rules for genetically modified organisms manipulation
  13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy
  14) New trends in biopharmaceutical products development
  

Literature

required literature
• Brawn. Klonování genů a analýza DNA. Univerzita Palackého v Olomouci,, 2007. info
• Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
• Ruml, T.et al. Genové inženýrství. VŠCHT Praha, 2002. info
• Ondřej, M., Drobník, J. Transgenoze rostlin. Academia Praha, 2002. info
• Vodrážka, Z. Biotechnologie. VŠCHT Praha, 1991. info

recommended literature
• Alberts et al. Základy buněčné biologie, Úvod do molekulární biologie buňky. Espero Publishing, 1998. info

not specified
• KAYSER, O., MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Ge, 2004. info
• HO, R. J. Y. , GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A, 2003. info
• SLATER, A., SCOTT, N., FOWLER, M. Plant biotechnology. The genetic manipulation of plants. Oxford University Press. Oxford, New York, U.S.A, 2003. info

Teaching methods

Monologic (reading, lecture, briefing)

Assessment methods

Oral exam
Written exam

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.

Teacher's information

the exem consists of two parts: written test (student has tu obtain at least 50% of of credits) and oral examination.

Activity	Difficulty [h]
Domácí příprava na výuku	12
Příprava na zkoušku	24
Účast na výuce	36
Summary	72

• Enrolment Statistics (Spring 2021, recent)
  
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