C7940 Bioanalytics I - Biomacromolecules

Faculty of Science
Autumn 2005
Extent and Intensity
2/0/0. 2 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
Chemistry Section – Faculty of Science
Timetable
Fri 8:00–9:50 Kontaktujte učitele
Prerequisites
Basic knowledge of biochemistry is prerequisited (C3181 Biochemistry I, C4182 Biochemistry II). C7895 Mass Spectrometry of biomolecules is a recommended lecture
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
Subject summarises the basic knowledge of the analytical approaches in analysis of biomacromolecules snd suprabiomolecular structures and also important theoretical background for their understanding.
Syllabus
  • - analytical approaches in v bioanalytics demands; high throuput, shotgun analysis, standardisation; affinity methods principles, conduct and use (separation, interaction studies), IMAC, APAGE
  • - immunoanalytic methods principles; immunoanalysis precipitation, agglutination, immunoessay, methods: FIA, LIA, RIA, EIA, ELISA, IAC, IACE, SELDI
  • - gel electrophoresis principles, conduct (1D, 2D); blotting
  • - genomics (analysis of DNA) fundaments of genetics (structura, function of DNA), analytical approaches: PCR, restriction enzymes; sequencing; DNA methylation and its analysis
  • - proteomics (analysis of proteins and their complexes) fundamentals of proteomics (structure of proteins and protein complexes); expression proteomics methodology, methods (2D HPLC, 2D PAGE, MS: bottom-up, top-down),
  • - functional proteomics - methodology, methods (TAP-tagging, Two-Hybrid, SEAM strategy)
  • - bioinformatics in MS-assisted proteomics identification of protein in organisms with and without sequenced genoms (MS BLAST, MultiTag)
  • - quantitative analysis of protein complexes internal standard method; mass tagging (ICAT, ICMT, TMT, MCAT, GIST, SILAC, SIL), absolute quantification of proteins and peptides by MS
  • - structural proteomics methodology (chemical cross-linking), methods (FTICR MS)
  • - lipidomics (analysis of lipids and cellular membranes) fundamentals in lipidomics (structure and function of cell. membranes and their components), analytical approaches: lipid extraction, TLC, GC, LC, SFC, GPC, MS (PIS, HGS, FAS)
Assessment methods (in Czech)
Přednáška je založena na prezentaci, která bude k dispozici ve formátu PDF. Předmět je ukončen ústní zkouškou.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008.
  • Enrolment Statistics (Autumn 2005, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2005/C7940