PřF:C8857 Study of interactions between - Course Information
C8857 Study of interactions between proteins and DNA
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 1/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Mgr. Lumír Krejčí, Ph.D. (lecturer)
- Guaranteed by
- doc. Mgr. Lumír Krejčí, Ph.D.
National Centre for Biomolecular Research – Faculty of Science - Timetable
- Thu 10:00–10:50 C04/211
- Prerequisites
- The course will be ended by test. Each student will have to also deliver home assignment that describes their particular project with the potential implementation of the methods and techniques discussed during the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biochemistry (Eng.) (programme PřF, N-BCH)
- Biochemistry (Eng.) (programme PřF, N-BCH3)
- Biochemistry (programme PřF, N-BCH)
- Biochemistry (programme PřF, N-BCH3)
- Biochemistry (programme PřF, N-CH)
- Biology (programme PřF, N-BI)
- Biomolecular Chemistry (Eng.) (programme PřF, N-BCH)
- Biomolecular Chemistry (Eng.) (programme PřF, N-BCH3)
- Biomolecular Chemistry (programme PřF, N-BCH)
- Biomolecular Chemistry (programme PřF, N-BCH3)
- Macromolecular Chemistry (programme PřF, N-CH)
- Macromolecular Chemistry (programme PřF, N-CH3)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, N-BI, specialization Genomika a proteomika)
- Molecular Biology and Genetics (programme PřF, N-BI, specialization Molekularni a geneticka diagnostika)
- Course objectives
- 1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
- Syllabus
- 1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
- Literature
- Assessment methods
- The course will be ended by test. Each student will have to also deliver home assignment that describes their particular project with the potential implementation of the methods and techniques discussed during the course.
- Language of instruction
- English
- Further Comments
- Study Materials
The course is taught annually.
- Enrolment Statistics (Autumn 2008, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2008/C8857