PřF:Bi9393c Anal.cytometry - Pract.course - Course Information

Bi9393c Analytical cytometry - practical course

Extent and Intensity

0/1. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

Mgr. Karel Souček, Ph.D. (seminar tutor)
Mgr. Zuzana Pernicová, Ph.D. (seminar tutor)
Mgr. Radek Fedr (seminar tutor)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10

Course objectives

Practical training in basic methods of flow cytometry and high speed sorting.

Syllabus

• Basic training in operation of routine flow cytometer FACSCalibur. Setup, calibration, quality control. Basic training in operation of high speed sorter. Setup, calibration, quality control. Cell cycle analysis. Immunophenotype analysis. GFP expression analysis and sorting. Data analysis - software FlowJo.

Literature

• Current Protocols in Cytometry, Print ISSN: 1934-9297 Online ISSN: 1934-9300

Teaching methods

practical training

Assessment methods

Evaluation of protocols.

Language of instruction

Czech

Follow-Up Courses

• Bi9393 Analytical cytometry

Further Comments

Study Materials
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• Bi9393cen Analytical Cytometry – Practical Course
  (!Bi9393c) && !NOWANY(Bi9393c) && NOW(Bi9393en)  

• Enrolment Statistics (Autumn 2011, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2011/Bi9393c