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Course Information

C9531 Structure of biomacromolecules

Faculty of Science
autumn 2012

Extent and Intensity

0/1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

Mgr. Olga Otrusinová (seminar tutor), doc. Mgr. Lukáš Žídek, Ph.D. (deputy)
Mgr. Pavel Srb, Ph.D. (seminar tutor)

Supervisor

doc. Mgr. Lukáš Žídek, Ph.D.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: doc. Mgr. Lukáš Žídek, Ph.D.
Dodavatelské pracoviště: National Centre for Biomolecular Research - Faculty of Science

Timetable

Tue 12:00–12:50 A4-118, Thu 9:00–9:50 A4-118, Thu 10:00–10:50 A4-118

• Timetable of Seminar Groups:

C9531/01
C9531/02
C9531/03

Prerequisites

NOW ( c9530 Structure of biomacromolecules )
The seminar is offered to students attending course C9530 (Structure of biomacromolecules).

Course Enrollment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

Fields of study the course is directly associated with

• Biochemistry (programme PřF, B-BCH)

Course objectives

The aim of the seminar is to offer the chance to practice the knowledge acquired in course C9530 (Structure of biomacromolecules) and to prepare students for the exam of the course C9530. Students attending the course will be able to analyze molecular structures, solving problems modeled as real examples from the laboratory practice, and combine results of various type of analyses to obtain a complex solution.

Syllabus

• 1. Torsion angle determination, work with protein models. 2. Work with ucleic acid models, determination of pseudorotation. 3. Program Rasmol, demonstration of molecular mechanics. 4. Nucleic acid sequencing, designing site-directed mutagenesis. 5. Peptide sequencing using mass spectrometry. 6. IR and CD protein spectra interpretation. 7. Sequential assignment of protein NMR spectra. 8. Sequential assignment of nucleic acid NMR spectra. 9. X-ray diffraction - resolution, phase problem, refinement. 10. Work with databases of sequences and structures.

Literature

• LESK, Arthur M. Introduction to protein architecture :the structural biology of proteins. New York: Oxford University Press, 2001. xii, 347 s. ISBN 0-19-850474-8. info
• Protein NMR spectroscopy :principles and practice. Edited by John Cavanagh. 2nd ed. Amsterdam: Elsevier, 2007. xxv, 885 s. ISBN 978-0-12-164491. info

Teaching methods

Practical problems are presented during the semester. After a detailed analysis of the model solution students demonstrate their ability to solve the probles individually in a form of tests.

Assessment methods

Students are required to solve at least 60% problems in each of oral test during semester. Excellent perforamce will be taken into account during the C9530 examination.

Further Comments

Study Materials
The course is taught annually.

Teacher's information

http://www.ncbr.chemi.muni.cz/~lzidek/C9531/C9531.html

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, autumn 2011, Autumn 2011 - acreditation, autumn 2013.

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• Enrollment Statistics (recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2012/C9531

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