Bi7900 Genetic methods in zoology

Faculty of Science
Autumn 2013
Extent and Intensity
2/1. 3 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Miloš Macholán, CSc. (lecturer)
prof. Mgr. et Mgr. Josef Bryja, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Miloš Macholán, CSc.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Miloš Macholán, CSc.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science
Timetable
Thu 8:00–10:50 BR4
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to give undergraduate and PhD students a basic orientation in principles of morphological, cytogenetic, and molecular methods currently used in zoological research. After finishing the course, students should gain theoretical knowledge and practical skills in the following topics: Analysis of phenotype (epigenetic traits, quantitative traits, geometric morphometrics); Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting“); Protein and DNA electrophoresis; DNA analysis (DNA isolation, DNA-DNA hybridization, PCR, real-time PCR, fingerprinting, RAPD, AFLP, analysis of microsatellites, LINE, SINE, and SNP; sequencing, "next generation sequencing, RFLP, DGGE, TGEE, SSCP, cloning, microarrays); Methods of analysis (population-genetic data: analyses based on allele frequencies vs. "individual-based" models; phylogenetic data: distance methods, maximal parsimony, maximal likelihood, Bayesian analysis).
Syllabus
  • 1. Analysis of phenotype (signal phenotypes, epigenetic traits, quantitative traits, landmark analysis)
  • 2. Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting") and electrophoretic methods (proteins, DNA)
  • 3. DNA analysis I (DNA isolation, genetic markers - nuclear vs. cytoplasmic DNA, DNA-DNA hybridization, PCR, real-time PCR, "multilocus" DNA markers: fingerprinting, RAPD, AFLP)
  • 4. DNA analysis II ("single-locus" DNA markers: microsatellites, LINE, SINE, SNP)
  • 5. DNA analysis III (sequencing methods, "next generation sequencing") 6. DNA analysis IV (SNP and their analysis: RFLP, DGGE, TGEE, SSCP, cloning, microarrays)
  • 7. Methods of analysis I (population-genetic data) - analyses based on allele frequencies vs. "individual-based" models
  • 8. Methods of analysis II (phylogenetic data) - distance methods, maximal parsimony, maximal likelihood, Bayesian methods
  • 9. Practical class I (chromosomal preparations, karyotype analysis)
  • 10. Practical class II (protein electrophoresis)
  • 11. Practical class III (PCR, real-time PCR)
  • 12. Practical class IV (analysis of DNA fragments after PCR, electrophoresis, fragment analysis, capillary SSCP)
  • 13. Practical class V (sequencing)
Literature
  • ZIMA, Jan. Genetické metody v zoologii. 1. vyd. Praha: Karolinum, 2004, 239 s. ISBN 8024607956. info
Teaching methods
lectures, practical classes
Assessment methods
lectures and laboratory courses; Each student will take an oral exam including at least 3-4 short inquiries from various areas specified in the syllabus. Emphesized are questions closely related to research activities of the student.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Autumn 2013, recent)
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