Bi2050c General microbiology practice

Faculty of Science
Spring 2009
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Mgr. Andrea Vávrová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Danuška Horáková, CSc.
Timetable of Seminar Groups
Bi2050c/01: Fri 8:00–10:50 Bpb,02012, M. Krsek, A. Vávrová
Bi2050c/02: Fri 11:00–13:50 Bpb,02012, M. Krsek, A. Vávrová
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
The main objective of the practical course of general microbiology is to introduce students into basic methods used in microbiological laboratory (methods of sterile work, inoculation and cultivation, microscopic slides preparation and examination of microorganisms, determination of the number of bacteria in different samples, methods of work with bacteriophage, yeasts and microscopic fungi).
Syllabus
  • 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of viable bacteria by plate count 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Determination of the number of phage particles in lysate of bacterial cells 10. Yeasts, test of vitality 11. Microorganisms in soil. Inoculation of microscopic fungi. 12. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 13. Microscopic fungi. 14. Reports, test and microscopic examination/determination of unknown bacterial strain.
Literature
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
Assessment methods
practical classes, reports, written test, credit
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/Bi2050c