PřF:Bi6150c Mycorrhizal symbioses - pr.

Bi6150c Mycorrhizal symbioses - practice

Faculty of Science
Spring 2009

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Milan Baláž, Ph.D. (seminar tutor)

Guaranteed by

Prerequisites (in Czech)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Course objectives

The aim of the practical course is to familiarize the students with basic methods of current mycorrhizal research.

Syllabus

Mycorrhizal colonization of plant roots: review of commonly used methods, assessment of root colonization (chemical and microscopic methods), observation of typical mycorrhizal structures.
Isolation of spores of VAM fungi: isolation techniques, stain by Melzer's reagent, sample preparation, spore description and fungal species determination.
Mycorrhizal extraradical mycelium: extraction techniques (membrane filtration and inserted membrane technique), vital staining techniques, assessment of extraradical hyphal length (grid-line intersect method and image analysis).
Long-term cultivation experiment: the aim of the experiment vary with every course run.

Literature

GRYNDLER, Milan, Milan BALÁŽ, Hana HRŠELOVÁ, Jan JANSA and Miroslav VOSÁTKA. Mykorhizní symbióza. O soužití hub s kořeny rostlin (Mycorrhizal symbiosis. On the coexistence of fungi with plant roots.). 1. vydání. Praha: Academia, 2004, 366 pp. 1. vydání. ISBN 80-200-1240-0. info
NORRIS, JR, DJ READ and AK VARMA. Techniques for the study of mycorrhiza, Part 1. London: Academic Press, 1991. info

Assessment methods

Active participation at the course,

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.

Teacher's information

http://elanor.sci.muni.cz/~mykorrhi