Bi6120 Plant Cell, Tissue, and Organ Culture

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (lecturer)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to:
describe basic principles of plant cell, tissue and organ culture in vitro;
explain the history and development of this cultures until today;
analyze the impact of different types of culture method on the plant genotype preservation and/or change;
propose in vitro methods suitable for plant multiplication, breeding and production.
Syllabus
  • Historical Background, Conditions of in vitro Culture, Laboratory Design.
  • Media Composition and Preparation. Plant Hormones and Growth Regulators.
  • In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture.
  • Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
  • Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
  • Test-tube Pollination/ Fertilization in vitro: Sterile Pollination of Isolated Placentae with Ovules. Fertiliation of Isolated Ovules and/or Izolated Gametes.
  • Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos.
  • Somatic Embryogenesis: Direct and Indirect Somatic Embryogenesis.
  • Ploidy reduction: Androgenesis: Initiation of Anther or Microspore Culture,Chromosome Doubling.
  • Plant Genome Transformation (GMO): Direct (Electroporation, Vacuum Infiltration, Biolistic Method and Indirect Methods (Disc Method of Agrobacterium-Mediated Transformation).
  • Cryopreservation: Rapid and Slow Freezing (Vitrification, Alginate Encapsulation/Dehydration Method).
  • Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
  • Indexing and Certification of in vitro Cultures
Literature
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • ŠEBÁNEK, Jiří and Zdeněk SLADKÝ. Biotechnologie rostlinných explantátů. 1. vyd. Brno: Vysoká škola zemědělská, 1988, 100 s. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • NOVÁK, František Jindřich. Explantátové kultury a jejich využití ve šlechtění rostlin. 1. vyd. Praha: Academia, 1990, 208 s. ISBN 8020003444. info
  • SEMAN, Ivan. Biotechnologické metódy v šl'achtení pol'ných plodín. 1. vyd. Bratislava: Príroda, 1990, 271 s. ISBN 80-07-00237-5. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • PIERIK, R. L. M. In vitro culture of higher plants. Dordrecht: Martinus Nijhoff Publishers, 1987. ISBN 90-247-3531-9. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
  • ONDŘEJ, Miloš. Genové inženýrství kulturních rostlin. 1. vyd. Praha: Academia, 1992, 232 s. ISBN 80-200-0310-X. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
  • Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
Teaching methods
Lectures.
Assessment methods
Written test or oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.