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Masaryk University

Faculty of Science

Master's degree programme/field of study:
Biology/Molecular Biology and Genetics

Mgr. Karla Kostýlková

Analysis of the etb gene-positive plasmid and study of its role in exfoliative toxin B production in Staphylococcus aureus

Analýza plazmidu obsahujícího gen etb a studium jeho role v produkci exfoliativního toxinu B u Staphylococcus aureus

Abstract: Cílem práce byla molekulární charakterizace etb-pozitivních plazmidů 10 vybraných kmenů Staphylococcus aureus izolovaných v České republice. Při řešení diplomové práce byla využita metoda REAP, PCR, klonování a bioinformatická analýza naklonované sekvence plazmidové DNA. Výsledky prokázaly, že se v souboru studovaných kmenů S. aureus vyskytují 4 různé typy etb-pozitivních plazmidů, z nichž jeden typ …more

Abstract: The objective of this study was molecular characterization of etb-positive plasmids from a representative set of 10 Staphylococcus aureus strains isolated in Czech Republic. Methods REAP, PCR, cloning and bioinformatic analysis of plasmid unknown sequence were used. Results confirmed that S. aureus strains studied in this work carry four different types of etb-positive plasmids. One type was found …more

Staphylococcus aureus epidermolýzy exfoliativní toxin B plazmidy REAP klonování horizontální přenos genů

Language used: Czech

  • Entered/Edited 17. 6. 2011 10:33, Marie Halasová
  • Record made 21. 12. 2009 13:46, Eva Nebolová
  • Accessible from: 16. 5. 2011 12:51, Marie Halasová
  • Thesis/dissertation received 16. 5. 2011 12:51, Marie Halasová

Master's thesis defence

  • Took place 9. 6. 2011, the thesis was defended successfully.

Supervisor:

  • doc. RNDr. Vladislava Růžičková, CSc., ÚEB Biol PřF MU, Department of Genome Research - Biology Section - Faculty of Science
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The thesis has been checked: 17. 5. 2011 10:02, doc. RNDr. Vladislava Růžičková, CSc.


Složka /th/  Závěrečné práce /th/
Složka /th/222753/  Mgr. Karla Kostýlková /222753/
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