Iresine herbstii – influence of light

Detector 254 nm

Chlorophyll – detector 450 nm

cultivation

           kaempferol-peak areas-fresh material

light

                                                                                                         115.5

dark

                                                                                                             0


cultivation

           chlorophyll- peak areas-fresh material

light

                                                                                                          30.1

dark

                                                                                                         148.5


Summary: On the first and second chromatogram we can see big differences in content of substances.
In the area of 10 minutes there are  several substances in Iresine grown in light conditions. One
of them is kaempferol glycoside. Iresine grown in darkness completely lacks these substances. Lack
of light causes at heliophilous plants reduced production of secondary metabolites.


Task: Calculate the relative concentration of kaempferol glycoside and chlorophyll in dry mass and
expressed it graphically.