LF:ABCHc Biochemistry-prac - Course Information

ABCHc Biochemistry - practice

Extent and Intensity

0/1/0. 1 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Mgr. Zdenka Fohlerová, Ph.D. (seminar tutor)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. David Galuška (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)

Guaranteed by

MUDr. Michaela Králíková, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

ABCHc/BPC1_BIO_01: Thu 15. 9. 8:00–9:40 A16/212, Thu 29. 9. 8:00–9:40 A16/212, Thu 13. 10. 8:00–9:40 A16/212, Thu 27. 10. 8:00–9:40 A16/212, Thu 10. 11. 8:00–9:40 A16/212, Thu 24. 11. 8:00–9:40 A16/212, Thu 8. 12. 8:00–9:40 A16/212, Z. Fohlerová
ABCHc/BPC1_BIO_02: Thu 15. 9. 8:00–9:40 A16/211, Thu 29. 9. 8:00–9:40 A16/211, Thu 13. 10. 8:00–9:40 A16/211, Thu 27. 10. 8:00–9:40 A16/211, Thu 10. 11. 8:00–9:40 A16/211, Thu 24. 11. 8:00–9:40 A16/211, Thu 8. 12. 8:00–9:40 A16/211, J. Víteček
ABCHc/BPC1_BIO_03: Thu 22. 9. 10:00–11:40 A16/216, Thu 6. 10. 10:00–11:40 A16/216, Thu 20. 10. 10:00–11:40 A16/216, Thu 3. 11. 10:00–11:40 A16/216, Thu 1. 12. 10:00–11:40 A16/216, Thu 15. 12. 10:00–11:40 A16/216, J. Kos
ABCHc/BPC1_BIO_04: Thu 22. 9. 10:00–11:40 A16/212, Thu 6. 10. 10:00–11:40 A16/212, Thu 20. 10. 10:00–11:40 A16/212, Thu 3. 11. 10:00–11:40 A16/212, Thu 1. 12. 10:00–11:40 A16/212, Thu 15. 12. 10:00–11:40 A16/212, Z. Fohlerová
ABCHc/BPC1_BIO_05: Thu 15. 9. 10:00–11:40 A16/212, Thu 29. 9. 10:00–11:40 A16/212, Thu 13. 10. 10:00–11:40 A16/212, Thu 27. 10. 10:00–11:40 A16/212, Thu 10. 11. 10:00–11:40 A16/212, Thu 24. 11. 10:00–11:40 A16/212, Thu 8. 12. 10:00–11:40 A16/212, Z. Fohlerová
ABCHc/BPC1_BIO_06: each odd Thursday 10:00–11:40 A16/216, J. Kos

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Biomedicínská technika a bioinformatika (programme LF, C-CV)

Course objectives

Subject is complementary to the Biochemistry lecture ABCHp. Students become familiar with the background and equipments of chemical laboratory. They will learn to perform simple laboratory operations. During experiments students verify some findings discussed in theoretical lessons.

Learning outcomes

After completing the course, the student will:
- be familiar with safety and health protection at work in the laboratory;
- be able to calculate the concentration of solutions, pipette, prepare or dilute a solution of a given concentration;
- be acquainted with the work with biological material and its processing in the laboratory of clinical biochemistry;
- be able to work with basic instruments in the biochemical laboratory, eg. pH meters, spectrophotometers;
- understand the principle and implementation of selected methods used in clinical biochemistry (optical, chromatographic, electrophoretic, immunochemical methods).

Syllabus

• Introductory information, safety rules. Preparing solutions of the given concentration. Biological material. Examinations in clinical biochemistry. Biochemical analyzers. The measurement of pH of various solutions. pH-metres. Spectrophotometry, absorption curve. Determination of glucose. Chromatography. Gel chromatography. Adsorption chromatography of plant pigments (silicagel). HPLC demonstration. Electrophoretic and immunochemical methods. Proteins in blood serum and urine. Electrophoresis of blood serum proteins. ELISA.

Literature

required literature
• Podklady pro cvičení v IS.
• Michaela KRÁLÍKOVÁ, Hana PAULOVÁ a kolektiv autorů. Biochemie pro biomedicínské techniky. 1. vyd. Brno: Masarykova univerzita, 2021. 177 s. ISBN 978-80-210-9857-2
• Podklady pro práci ve cvičení jsou uloženy v interaktivní osnově na IS MUNI.

Teaching methods

homework theoretical preparation, practical exercises, presentation of laboratory methods and instruments.

Assessment methods

Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports is the condition for the course-unit credit. Obtaining of course-unit credits of practicals is the pre-requisite for registration to the examination.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 15.

Teacher's information

Laboratory coat, laboratory gloves and overshoes are necessary, as well as a FFP2 respirator without exhalation valve according to current covid regulations. Attendance in practicals is obligatory. All absences must be justified through the Department of study affairs. Illness or some other serious reason are usually the only acceptable excuse for absence from lesson and must be officially confirmed. After being absent the student must make up for the lesson. Absences that are not apologized are not allowed to make up. Student is obliged to prepare himself for each lesson. Students that are not sufficiently prepared will not be admitted in participating in the lesson.

• Enrolment Statistics (autumn 2022, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2022/ABCHc