BLIT063 Laboratory Instrument and Analytic Technology

Faculty of Medicine
Spring 2009
Extent and Intensity
0/0/0. 5 credit(s). Type of Completion: SZK (final examination).
Teacher(s)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Prerequisites (in Czech)
BLIT0122p Instrument Technology II - l. && BLBS051p Biostatistics - lecture && BLZP051p Environment monitoring
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of the theoretical preparation part is to provide a theoretical base of physical principles used in analytical and laboratory technology. To familiarize students in detail with the application of these physical principles in specific instruments including existing modifications in contemporary medical laboratory equipment. To observe variations and modifications of specific examples and determine their purpose, advantages and critical sections in seminars, demonstrations and practical exercises.
Syllabus
  • Basic physical principals used in instrumental technology; Optical methods (spectrophotometry, turbidimetry, fluorimetry, nephelometry, denzitometry, refractometry, reflectance photometry, phlame emission photometry, atomic absorption spectrophotometry; Electrochemical methods (potentiometry, amperometry, polarography, coulometry, conductometry, electrophoresis); Ion-selective electrodes (ISE); Kryoscopy, ebullioscopy; Microscopy; Radioactive isotopes; Centrifugation; Basic physical principals used in instrumental technology – Demonstration; Spectrophotometer-design (sources of lightradiation, monochromatores, interference filtrs, cuvettes, detectors; Phlame emission photometer -design, sampling of analytical sample, internal standard; pH meter; Polarograph; Coulometer; Conductometer; Denzitometer; Application of basic physical principals in laboratory instrumentation; Spectrophotometer UV/VIS, fluorimeter, nephelometer, denzitometer, refractometer, reflectance photometer, phlame emission photometer, atomic absorption spectrophotometer; pH meter, polarograph, coulometer, conductometer; electrophoretic device (agarose, polyakrylamid, isoelectric focusation); Thin layer chromatography device, liquid chromatograph-HPLC, gas chromatograph-GC; Osmometer; ISE (Na, K, Cl, Ca, Mg); Clarck electrode (pO2), Severinghaus electrode (pCO2 ), enzymatic electrodes; Centrifuges; Termostats, incubation bathes; Microwave mineralization device; Devices for RIA; Luminometer;
Literature
  • DASTYCH, Milan. Instrumentální technika : obor zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2007, 131 s. ISBN 9788021042261. info
Assessment methods
final state examination
Language of instruction
Czech
The course is also listed under the following terms Spring 2008, Spring 2010, Spring 2011.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/med/spring2009/BLIT063