BLKHM051x Histopathological Techniques - practice

Faculty of Medicine
spring 2020
Extent and Intensity
0/0/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
Michaela Gregorovičová (assistant)
Guaranteed by
PhDr. Květoslava Matulová
Department of Pathology – Joint workplaces with the University Hospital Brno - workplaces of the Bohunice and Mater. Hospital – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Pathology – Joint workplaces with the University Hospital Brno - workplaces of the Bohunice and Mater. Hospital – Faculty of Medicine
Prerequisites
BLKHM0422c Histopath. tech. II
The student have to have completed subjects Histopathological examination methods I, II.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Specialist practice complements the practical part of education in Clinical Biochemistry, Clinical Hematology, Immunohematology and Transfusion medicine, Clinical Microbiology, Clinical Immunology, Clinical Genetics and Pathologic Anatomy – Histopathologic determination methods. Students become familiar with the organization of workflow processes and basic documentation of laboratory worksites and will perform select tasks and activities.
Learning outcomes
Student will be able: - to prepare formalin-fixed and paraffin-embedded tissue blocks - to cut the tissue slices and place them on to glass microscope slides - to stain the tissue sections by routine hematoxylin and eosin staining - to stain the tissue sections by special staining - perform immunohistochemistry - perform PCR - to process cytology samples
Syllabus
  • Reception office in pathologic department, administration. Reception laboratory for bioptic examination, biopsy during operation. Laboratory with tissue processors, blocking, embedding of tissues. Standard histopathologic laboratory. Special histopathologic laboratory I and II. Rooms for autopsy, manipulation necroptic material. Laboratory for immunohistochemistry. Laboratory for histochemistry. Laboratory for molecular pathology.
Literature
  • LUKÁŠ, Zdeněk, Iva KROUPOVÁ, Martin FALK, Iveta VALÁŠKOVÁ, Lenka FAJKUSOVÁ and J SEDLÁČKOVÁ. RNA fluorescence in situ hybridization analysis of fetal tissues from a family affected by myotonic dystrophy. Neuromuscular Disorders. 2007, 17/2007, No 10, p. 856. ISSN 0960-8966. info
  • PAVLOVSKÝ, Zdeněk, Zdeněk LUKÁŠ, Marie KUNČÍKOVÁ and Andrea ZAKOUTOVÁ. Kongenitální myopathie s hypoplazií vláken 2. typu. Československá patologie a soudní lékařství. 2001, vol. 37, No 2, p. 69-71. ISSN 1210-7875. info
  • LUKÁŠ, Zdeněk. Imunohistochemické metody v biologii a v bioptické diagnostice : Imunohistochemical methods in biology and surgical pathology. 1. vyd. Brno: Masarykova univerzita, 1997, 147 s. : b. ISBN 80-210-0620-X. info
  • LUKÁŠ, Zdeněk and Josef FEIT. Úvod do bioptické myopatologie. 1. vyd. Brno: Masarykova univerzita, 1996, 114 s. ISBN 80-210-1294-3. info
Teaching methods
practice in labs of the department of pathology
Assessment methods
credit, full attendance is necessary
Language of instruction
Czech
Further comments (probably available only in Czech)
General note: Všechny formy kontaktní výuky předmětu vyjma přednášek jsou povinné.
Information on the extent and intensity of the course: 40.
The course is also listed under the following terms Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019.
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