BLKMT0311p Toxicological Techniques - lecture

Faculty of Medicine
Autumn 2011
Extent and Intensity
0/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
Mgr. Andrea Brzobohatá, Ph.D. (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
Mgr. Andrea Brzobohatá, Ph.D.
Department of Forensic Medicine – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: Mgr. Andrea Brzobohatá, Ph.D.
Prerequisites (in Czech)
BLKLC011s Med.Chem.-seminary
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this subject is to acquaint students with principles of toxicological examining methods used in clinical, industrial, hygienic and pharmacological toxicology. They are physiochemical methods used for proof and determination of toxic substances, medicaments, eventually their metabolites in stomach and intestine content, in bodily secretions and excretions, in all bodily fluids and organs with the aim to assess the cuase of poisoning.
Syllabus
  • Toxicity of the substance; Principles of the Good laboratory practice; Principles of the proper sampling, transporting and preservation of biological symplex; Metabolic pathways of substances, pharmacokinetics and pharmacodynamics ; Biological material treatment for toxicological analysis (types of the biological material, sensory examination, deproteinisation, isolation – elimination of balast substances, transfer to the organic phase, preconcentration, diffusion methods, mineralisation, vapour distillation, atc.); Volatile substances, determination of ethanol, head-space – methods, metabolites; Inorganic substances, acids, bases, metalic substances, cyanides, carbon monooxide; Extractable substances, medicaments, drugs of abuse, agrochemicals, evidence; Principles of the isolation (separation) methods (extraction liquid-liquid, solid-phase extraction, microextraction, effect of pH, hydrolysis); Methods of isolation of unknown noxa (toxic, narcotic and psychotropic substances in biological material); Principles of immuno-assay tests for proof of unknown toxic substance (Systematic toxicological analysis, immunochemistry, TLC, GC/MS, HPLC); Principles of substance identification, GC/MS, derivatization, target analysis; Principles of methods for determination of concentrations of medicaments, volatile toxic substances, narcotic and psychotropic substances (GC, HPLC, CE, specific detectors, standards, atc.); Biological and exposure tests (testing of substance toxicity, testing on animals, determination of noxa metabolites in urine, resp. in blood related to exposition to some substances in workplace; Profession testing, cut-off – values; Alternative materials (hair, saliva, sweat, meconium); Biological proof of toxic substance; Interferences in toxicological practice (immunochemistry, cross-reactivity, physiological levels of endogenous substances: hippuric acid, urea, foodstuff additives, phthalates, disinfection artefacts, poppy diet); Internal and external quality control in toxicology (validation of methods, precision, accuracy, reproducibility, cut-off – values, internal standards, comparative blank samples, skill testing – unknown sample, proof by two independent methods at least); Laboratory calculations in toxikology; Statistical data treatment in toxikology; Fundamentals of method establishment in toxicology ;
Literature
Teaching methods
lecture
Assessment methods
examination
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 10 h/semestr.
The course is also listed under the following terms Autumn 2010, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2011/BLKMT0311p