LF:BLLC0111c Medical Chemistry - practice - Course Information
BLLC0111c Medical Chemistry - practice
Faculty of Medicineautumn 2025
- Extent and Intensity
- 0/3/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching - Teacher(s)
- Mgr. David Galuška (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Iva Audy (assistant)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant) - Guaranteed by
- doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine - Prerequisites
- Participation in the course is conditional on the successful completion of the course ZC011.
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Medical Laboratory Technologist (programme LF, B-LABD)
- Course objectives
- The main objectives of the course are to become oriented in the environment of the chemical laboratory and its equipment, to acquire simple laboratory procedures and techniques, to practice common separation procedures and become familiar with handling standard laboratory machines, to teach students how to process measurement results into protocols, evaluate them and draw conclusions.
- Learning outcomes
- Upon completion of the course, the student will be able to
- understand the most important bioanalytical principles
- manipulate basic laboratory tools and equipment
- pipetting and weighing according to the principles of good laboratory practice
- prepare a solution at a given concentration
- measure the pH of solutions, titrate
- prepare a suitable buffer and determine its buffering capacity
- master basic separation techniques - filtration, dialysis, centrifugation, extraction, chromatography
- perform basic qualitative analysis - precipitation, colour reactions
- apply spectrophotometry for bioanalytical purposes - Syllabus
- Safety and health protection when working in the laboratory, first aid principles.
- Measuring containers/tools. Heating, cooling, weighing. Pressure bottles.
- Measuring small volumes of liquids, pipettor calibration.
- Preparation of solutions of a given concentration.
- Filtration, dialysis, ultrafiltration, centrifugation. Concentration of samples.
- Measuring pH of solutions, calibration of pH meters.
- Neutralization volumetric analysis. Titration neutralization curves.
- Preparation of buffers, determination of buffer capacity.
- Ionic reactions, ion-selective electrodes, precipitation reactions.
- Chromatography.
- Spectrophotometry.
- Oxidation of alcohols, evidence of aldehydes/ketones, color reactions of amino acids.
- Protein solubility, denaturation, methods of protein quantification.
- Literature
- required literature
- Interaktivní osnova v IS MU.
- Teaching methods
- Practical exercises.
- Assessment methods
- Course-unit credit. Conditions for giving course-unit credit:
1) Completion of all exercises, incl. submission of all lab reports and 2) Completion of progress tests. - Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught every week. - Listed among pre-requisites of other courses
- Teacher's information
- A white coat, slippers or sturdy shoes with overshoes, gloves, and a calculator are required for the practical class.
Detailed information on the teaching and completion of the course is given in the Study Materials in IS MU.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/med/autumn2025/BLLC0111c