EMMB0411p Methods of molecular biology

Faculty of Medicine
spring 2022
Extent and Intensity
3/0/0. 5 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
EMBI0222p Biologie II - přednáška || EMBI0222s Biologie II - seminář
Basic knowledge of the structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for the study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of the principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand the principles of the methods and manage to choose suitable methodological approaches to address specific biological phenomena at the molecular level.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 45.
The course is also listed under the following terms spring 2023, spring 2024.
  • Enrolment Statistics (spring 2022, recent)
  • Permalink: https://is.muni.cz/course/med/spring2022/EMMB0411p