EMMT0411s Mikroskopické techniky - seminář

Faculty of Medicine
spring 2023
Extent and Intensity
0/2/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. MVDr. Aleš Hampl, CSc. (lecturer)
Mgr. Zuzana Sumbalová Koledová, Ph.D. (lecturer)
Guaranteed by
Ing. Josef Jaroš, Ph.D.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Timetable
Mon 11:00–12:40 F01B1/126
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to explain principles of microscopy, methods of tissue processing and image analysis. Student will get the understanding about wide spectrum of microscopic techniques and their application. Student will learn methods of processing tissue samples – cells, tissues for individual microscopic techniques. Finally, student will be able to choose optimal method for detection of specific cell structures and molecules, able to explain and perform some of methods of processing of tissue samples for histological analysis with light microscopy.
Learning outcomes
Student will:
- understand wide spectrum of microscopic techniques and their application.
- know methods of processing tissue samples – cells, tissues for individual microscopic techniques.
- be able to choose optimal method for detection of specific cell structures and molecules
- be able to explain and perform some of methods of processing of tissue samples for histological analysis with light microscopy.
Syllabus
  • 1. Fundamentals of light microscopy – transmission light, dark field, contrast enhancement, stereomicroscopy, image acquisition
  • 2. Preparation of biological samples. Principles of histological staining.
  • 3. Fluorescence microscopy – epifluorescence, confocal, holographic, spectral microscopy, SPIM.
  • 4. Superresolution microscopy – SIM, STED, PALM, STORM.
  • 5. Live imaging of cells. Techniques. Staining of samples.
  • 6. Automated high content screening microscopy.
  • 7. Electron microscopy – principles, sample preparation, SEM, TEM, CryoTEM, SBF-SEM.
  • 8. Special microscopy – 2-photon, AFM, X-ray microscopy, microCT.
  • 9. Image processing and analysis.
Literature
    recommended literature
  • Cell imaging techniques: methods and protocols. Edited by D:J. Taatjes, B.T. Mossman. Humana Press, 2006.
  • Theory and practice of histological techniques. Edited by John D. Bancroft - Marilyn Gamble. 5th ed. Edinburgh: Churchill Livingstone, 2003. xiii, 796. ISBN 0443064350.
Teaching methods
seminar
Assessment methods
written examination
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms spring 2022, spring 2024.
  • Enrolment Statistics (spring 2023, recent)
  • Permalink: https://is.muni.cz/course/med/spring2023/EMMT0411s