LF:MBKM071p Clinical microbiology II - lec - Course Information

MBKM071p Clinical microbiology II - lectures

Extent and Intensity

2/0/0. 4 credit(s). Type of Completion: zk (examination).

Teacher(s)

doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Mgr. Petra Myšková, Ph.D. (lecturer)
MUDr. Ivana Vítková (lecturer)
prof. RNDr. Lenka Zdražilová Dubská, Ph.D. (lecturer)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
Michaela Gregorovičová (assistant)

Guaranteed by

MUDr. Ivana Vítková
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Timetable

Tue 13. 9. 9:00–11:30 B11/228, Tue 20. 9. 9:00–11:30 B11/228, Tue 27. 9. 9:00–11:30 B11/228, Tue 4. 10. 9:00–11:30 B11/228, Tue 11. 10. 9:00–11:30 B11/228, Tue 18. 10. 9:00–11:30 B11/228, Tue 25. 10. 9:00–11:30 B11/228, Tue 1. 11. 9:00–11:30 B11/228, Tue 8. 11. 9:00–11:30 B11/228, Tue 15. 11. 9:00–11:30 B11/228

Prerequisites

Knowledge of microbiology at the level of Bachelor´s degree program.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Bioanalytical Laboratory Diagnostics in Medicine - Bioanalyst (programme PřF, N-BIA)

Course objectives

The subject of Medical Microbiology is based on the basics acquired by students in the Bachelor's degree program.
The aim of the theoretical part is to provide extensive information on the properties of medically important microorganisms and pathogenesis, clinic, epidemiology and therapeutics of infectious diseases and detailed information on microbiological investigation procedures, principles of indication of microbiological examinations, proper collection and delivery of infectious material and correct interpretation of the results with respect to the clinical condition of the patient including the possibility of antibiotic therapy. The practical part of the preparation is to gain practical experience and skills in the analysis of biochemical properties, in antigenic, proteomic and molecular biological analysis.
Upon completion of the course the student will be oriented in detailed diagnosis of individual etiological agents of infectious diseases: bacteria, viruses, parasites and yeasts and fungi. The student will be able to diagnose specific etiological agents based on detailed knowledge of biochemistry, antigenic structure, protein composition, and molecular biological properties of microorganisms. Will be acquainted with both basic and specialized microbiological procedures, including knowledge of apparatuses available in current laboratory microbiological practices. Will be able to interpret the result of the laboratory examination. Will be trained in combination skills, understanding the wider context of infectious diseases. Will be able to focus on the issue of nosocomial infections. Will know the principles of rational antibiotic therapy and antibiotic policy at national and international level. Will be familiar with the principles of quality management in medical microbiology.

Learning outcomes

Upon completion of the course the student will be oriented in detailed diagnosis of individual etiological agents of infectious diseases. The student will be able to diagnose specific etiological agents based on detailed knowledge of biochemistry, antigenic structure, protein composition, and molecular biological properties of microorganisms. Will be acquainted with both basic and specialized microbiological procedures, including knowledge of apparatuses available in current laboratory microbiological practices. Will be able to interpret the result of the laboratory examination. Will be able to focus on the issue of nosocomial infections. Will know the principles of rational antibiotic therapy and antibiotic policy at national and international level.

Syllabus

• Theoretical part:
• Introduction to clinical microbiology, history of microbiology
• Methods of microscopy, colouring procedures in microbiology
• Cultivation methods, preparation of cultivation soils
• Biochemical methods of bacterial identification
• Immunological methods for the identification of micro-organisms
• Proteomic methods of bacterial identification
• Molecular Biological Methods for Identification of Microorganisms
• Immunology (specifics of antimicrobial immunity)
• Epidemiology of individual infections (spread of infectious agents, transmission routes, epidemic investigations and anti-epidemic measures)
• Nosocomial infections, their prevention and prophylaxis
• Principles of antibiotic therapy, antibiotic policy
• Computing in microbiological laboratories
• Special theoretical part:
• Identification of individual micro-organisms species or groups thereof using specific biochemical, immunological, proteomic and molecular biological methods:
• Enterobacteria (salmonella, shigella, escherichia, etc.), vibria and campylobacter
• Gram-negative non-fermenting rods and cocobacilli (pseudomonads, burkholderie, acinetobaktery, legionella.)
• Cultivatingly demanding gramnegative rods (haemophilus, bordeles, francisels, brucellas, etc.)
• Gram-negative cocci (neisserie, moraxelly, branhamelly, etc.)
• Gram-positive cocci (staphylococci, streptococci, enterococci, etc.)
• Gram-positive aerobic rods (bacilli, listeria, corynebacteria, nokardia)
• Anaerobic microbes (clostridia, actinomycetes, peptostreptococci, bacteroids, etc.)
• Mycobacteria
• Spirochetes
• Mycoplasmata
• Rickettsie, ehrlichie, chlamydia etc.
• Yeast (candida, cryptococcus, pneumocyst) and mold (aspergillus, dermatophyte)
• RNA-viruses (picornaviruses, rotaviruses, orthomyxoviruses, paramyxoviruses, rhabdoviruses, retroviruses, togaviruses)
• DNA viruses (papillomaviruses, adenoviruses, hepadnaviruses, herpesviruses, poxviruses) and prions
• Pathogenic protozoans (trichomonads, lamblie, toxoplasms, plasmodia, leishmania, trypanosomes), multicellular endoparasites (Trematoda, tapeworms, nematodes) and ectoparasites (lice, fleas,
• Clinical microbiology (common microflora of the individual organ systems, the most common pathogens in individual localizations), the relationship between the microbiological finding and the clinical condition of the patient.
• Theoretical part methodical:
• Principles of microscopic techniques
• Principles of colouring techniques
• Cultivation techniques: use of individual cultivation soils, utilization of different atmospheric composition for cultivation, utilization of different growth

Literature

• JURÁNKOVÁ, Jana. Klinická mikrobiologie v laboratorní praxi : bakalářský obor Zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2011, 73 s. ISBN 9788021056572. info
• VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody (Medical Microbiology: Laboratory Procedures). 1st ed. Brno: Neptun, 2010, 495 pp. ISBN 978-80-86850-04-7. info
• VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology. 2nd revised ed.). 2. přepracované vydání. Brno: NEPTUN, 2005, 351 pp. ISBN 80-86850-00-5. info
• VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1st ed. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003, 495 pp. ISBN 80-902896-6-5. info

Teaching methods

lectures with help of audiovisual aids (.ppts, commented videos) with interactive input

Assessment methods

oral examination

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
Information on the extent and intensity of the course: 30.

• Enrolment Statistics (autumn 2022, recent)
  
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