aVLBC0321p Biochemistry I - lecture

Lékařská fakulta
podzim 2021
Rozsah
3/0/0. 45. 3 kr. Ukončení: z.
Vyučováno prezenčně.
Vyučující
doc. RNDr. Jiří Dostál, CSc. (přednášející)
prof. RNDr. Eva Táborská, CSc. (přednášející)
doc. RNDr. Josef Tomandl, Ph.D. (přednášející)
MUDr. Michaela Králíková, Ph.D. (pomocník)
RNDr. Hana Paulová, CSc. (pomocník)
Mgr. Jiří Slanina, Ph.D. (pomocník)
Bc. Ivana Ševčíková (pomocník)
Mgr. Marie Tomandlová, Ph.D. (pomocník)
Garance
doc. RNDr. Josef Tomandl, Ph.D.
Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Kontaktní osoba: prof. RNDr. Eva Táborská, CSc.
Dodavatelské pracoviště: Biochemický ústav – Teoretická pracoviště – Lékařská fakulta
Rozvrh
Po 8:00–9:50 B11/114, St 15:00–15:50 B11/114
Předpoklady
aVLBI0222p Lékařská biologie II - př && aVLBF011p Biophysics - lecture
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. Understanding these processes is a base for comprehension of metabolism on the tissue and organ level. In the introductory lessons are summarized basic terms from chemistry needed for an understanding of body structure physico-chemical processes occurring in it (chemical composition of the body, survey of biologically important elements, water, electrolytes, non-electrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemical pathways in cells.
Výstupy z učení
At the end of the course students will:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- describe the role of macro-and microbiogenic elements in the organism
- discuss the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism, and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications.
- understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.
Osnova
  • Chemical composition of the human body, biogenic elements. Water (properties, distribution), electrolytes, non-electrolytes, osmolality.
  • Acid-base reactions, pH, buffers, Henderson-Hasselbalch equation.
  • Bioenergetics (Gibbs energy, high-energy compounds, chemical equilibrium). Introduction to metabolism.
  • Redox reactions (redox potential, Nernst-Peters equation, biological redox systems).
  • Enzymes (classes, nomenclature). Characteristic features, enzyme kinetics, inhibition.
  • Cofactors (overview, functions, relation to vitamins).
  • Three phases of catabolism, citrate cycle, respiratory chain, oxidative phosphorylation.
  • Saccharides (structures), saccharides in nutrition, digestion.
  • Metabolism of glucose, transport of glucose into cells, glycolysis (aerobic, anaerobic). Pyruvate dehydrogenase complex. Gluconeogenesis.
  • Glycogen (synthesis, catabolism).
  • Metabolism of fructose, galactose, lactose. Pentose phosphate pathway. Glycosaminoglycans, proteoglycans.
  • Lipids and fatty acids (classification, structure). Lipids in nutrition. Lipophilic vitamins.
  • Digestion of lipids, surfactants. Fatty acids (structure, synthesis, catabolism).
  • Triacylglycerols (synthesis and degradation). Ketone bodies (synthesis, catabolism), phospholipids (structural types, synthesis, catabolism).
  • Lipid peroxidation (reactions, products, antioxidants).Eicosanoids (main features of synthesis). Cholesterol (structure, synthesis, regulation, conversions).
  • Bile acids (structure, synthesis, conversions). Calciols (structure, synthesis, conversions).
  • Amino acids (structure, properties). Peptides. Proteins (structure, folding, properties, structure of myosin, collagen, immunoglobulins).
  • Hemoglobin, myoglobin (structure, functions, Bohr effect), hemoglobinopathies.
  • Digestion of proteins, intracellular degradation of proteins.
  • General features in the metabolism of amino acids. Ammonia – sources, transport forms, detoxication.
  • Catabolism of individual amino acids. Congenital disorders of amino acids metabolism.
  • Synthesis of non-essential amino acids.
  • Conversions of amino acids to specialized products (synthesis of biogenic amines, creatine, NO, melanin, iodothyronines, carnosine).
  • Synthesis of heme, porfyrias.
  • Structure of nucleosides and nucleotides, uric acid. Synthesis and catabolism of purine and pyrimidine bases, synthesis of nucleotides.
  • Structure of DNA, replication, repairs.
  • Structure of RNA, transcription and its regulation, processing.
  • Synthesis of proteins, folding. Transport and sorting of proteins, post-translational modifications.
  • Biosynthesis of collagen, elastin, proteoglycans.
Literatura
    povinná literatura
  • RODWELL, Victor W., David A. BENDER a Kathleen M. BOTHAM. Harper's illustrated biochemistry. 30th ed. New York: Mc Graw- Hill, 2015, xii, 817. ISBN 9781259252860. info
  • KOOLMAN, Jan a Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
    doporučená literatura
  • LIEBERMAN, Michael, Allan D. MARKS a Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 4th ed. Baltimore: Lippincott, Williams & Wilkins, 2013, ix, 1014. ISBN 9781451100037. info
  • VASUDEVAN, D. M., S. SREEKUMARI a Kannan VAIDYANATHAN. Textbook of biochemistry for medical students. Eighth edition. New Delhi: Jaypee The Health Sciences Publisher, 2016, xvi, 718. ISBN 9789385999741. info
  • RONNER, Peter. Netter's Essential Biochemistry. Philadelphia: Elsevier, 2018, 482 s. ISBN 978-1-929007-63-9. info
Výukové metody
Lectures.
Metody hodnocení
Students pass to continuing education in next semester without any duty of knowledge checking in this semester.
Vyučovací jazyk
Angličtina
Další komentáře
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Předmět je zařazen také v obdobích podzim 2016, podzim 2017, podzim 2018, podzim 2019, podzim 2020, podzim 2022, podzim 2023.