aVLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2025
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
Miriam Minaříková (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: MUDr. Michaela Králíková, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
aVLBC0321s Biochemistry I - seminar && aVLFY0321c Physiology I - practice && aVLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to aVLBC0422p. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
The student will be able:
- to explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation.
- to describe physiological values of the most important biochemical parameters.
- to interpret the results of biochemical analyzes, discusses the significance of the findings.
- to understand the principles of the most important biochemical-analytical methodologies.
- to be oriented in basic laboratory manipulations.
Syllabus
  • Introduction to practice - organization, work and safety principles, principles of pipetting
  • Selected examinations of glucose metabolism - P/B/U-glucose, glycemic profile, OGTT, U-ketone bodies
  • Selected examinations of lipid and cholesterol metabolism - S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol
  • Selected liver tests - ALT/AST, S/U-bilirubin, U-UBG
  • Investigation of proteins in blood serum - S-total protein, S-albumin, electrophoresis, S/B-CRP, S-ferritin
  • Selected kidney examinations - S/U-creatinine, creatinine clearance, eGFR, FR and FE water, U-proteins, U-albumin, S/U-urea, nitrogen balance
  • Chemical qualitative analysis of urine - diagnostic strips
  • Selected examinations of urolithiasis - S/U-uric acid, stones
Literature
    required literature
  • Study materials for the course in IS MU.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes, missed exercises must be made up,
  • elaboration and approval of all protocols.
    The detailed conditions are posted in IS MU.
    • Language of instruction
    English
    Further comments (probably available only in Czech)
    The course is taught annually.
    The course is taught: every week.
    General note: Successful completion of the course is a condition for access to the exam in the course aVLBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MU in the Course-related instructions.
    A white coat, slippers or sturdy shoes with overshoes, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024.
    • Enrolment Statistics (spring 2025, recent)
    • Permalink: https://is.muni.cz/course/med/spring2025/aVLBC0422c