F1IA1_13 Instrumental Analytical Methods in Pharmaceutical Technology

Faculty of Pharmacy
Autumn 2024
Extent and Intensity
1/1/0. 3 credit(s). Type of Completion: zk (examination).
Taught in person.
Teacher(s)
doc. Mgr. Jan Muselík, Ph.D. (lecturer)
Mgr. Sylvie Pavloková, Ph.D. (lecturer)
doc. Mgr. Jan Muselík, Ph.D. (seminar tutor)
Mgr. Sylvie Pavloková, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Jan Muselík, Ph.D.
Department of Pharmaceutical Technology – Departments – Faculty of Pharmacy
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
The subject named "Instrumental analytical methods in the pharmaceutical technology" is dealing with modern instrumental methods useful in the field of the pharmaceutical technology, development and industry. The main aim of this subject is to create a comprehensive overview of methods used for the evaluation, analysis and quality control of pharmaceutical dosage forms. Main topics: Near and middle infrared spectroscopy, Raman microscopy, dissolution tests and drug content analysis (UV/VIS spectrophotometry, HPLC), light and electron microscopy, laser diffraction analysis, X-ray diffraction analysis, thermal analysis. The theoretical principles of selected methods are discussed and their practical applications are demonstrated.
Learning outcomes
After completing the course, the student will be able to: - summarize the main methods used in the analysis of solid dosage forms; - understand the theoretical basis of these methods; - describe the main applications of these methods in pharmaceutical research and industry.
Syllabus
  • 1 Middle and near infrared spectroscopy, principles, instrumentation, measurement techniques.
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    2 Applications of infrared spectroscopy, evaluation of spectra
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    3 Dissolution test, methods of determination of active compound content (UV/VIS spectrophotometry).
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    4 Methods of determination of active compound content (HPLC).
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D
    5 Division of modern microscopic methods; light microscopy: basics of optics, resolution, and magnification of the microscope, lens defects, microscope design, sample preparation, basic methods, measurement principles, use in pharmaceutical technology.
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    6 Electron microscopy: interaction of electrons with the matter, electron source, microscope construction, sample preparation, basic methods (scanning, transmission, and environmental electron microscopy), use in pharmaceutical technology.
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    7 Scanning electron microscopy: standard operating procedure, the influence of parameters on image quality, practical examples.
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    8 Scanning probe microscopy: scanning tunneling microscopy and atomic force microscopy (basic principles, microscope design, measurement modes, use in pharmaceutical technology).
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    9 Determination of particle size and shape: parameters, basic methods (sieve analysis, sedimentation analysis, Coulter particle counters, microscopic techniques, image analysis).
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    10 Methods of particle size determination - laser diffraction
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    11 X-ray analysis, instrumentation and applications in pharmaceutical technology.
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    12 Methods of thermal analysis (differential thermal analysis - DTA, thermogravimetry - TGA, differential scanning calorimetry - DSC).
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    13 Raman microscopy.
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    14 Tutorial giving

    SYLABUS OF LABORATORY PRACTISE
    1.-2. FT-NIR (evaluation of spectra by chemometric methods).
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    3.-4. Light microscopy in connection with image analysis: work with the device and software, analysis of a model pharmaceutical sample - determination of basic size and shape parameters.
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    5.-6. Optimization of chromatographic conditions, dissolution test of dosage form, chromatographic determination of active compound content.
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    7.-9. Electron microscopy: work with instrument and software, analysis of a model pharmaceutical sample, examples of use in research at DPT.
    Lecturer: Mgr. Sylvie Pavloková, Ph.D.
    10.-11. Thermal analysis. Calculations.
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
    12.-13. Excursion to the laboratory of Raman microscopy.
    Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
Literature
    required literature
  • Muselík J. Vybrané instrumentální metody ve farmaceutické technologii. Brno, 2012. ISBN 978-80-7305-640-7. info
Teaching methods
lectures laboratory practice
Assessment methods
Written test
Language of instruction
Czech
The course is also listed under the following terms Autumn 2020, Autumn 2021, Autumn 2023.
  • Enrolment Statistics (Autumn 2024, recent)
  • Permalink: https://is.muni.cz/course/pharm/autumn2024/F1IA1_13