F1SP1_14 Pharmacogenomics - practical classes

Faculty of Pharmacy
Autumn 2024

The course is not taught in Autumn 2024

Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
PharmDr. Mgr. Alžběta Kružicová, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Mgr. Alžběta Kružicová, Ph.D.
Department of Pharmacology and Toxicology – Departments – Faculty of Pharmacy
Prerequisites (in Czech)
FAKULTA(FaF) || OBOR(MUSFaF)
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to enable students to gain practical knowledge of basic molecular-biological methods used in pharmacogenomics and pharmacogenetics (DNA isolation, PCR method, restriction cleavage, agarose gel electrophoresis, etc.).
Learning outcomes
At the end of the course, the student will be able to identify and summarize important features of the main methods used in pharmacogenomics and pharmacogenetics. Will be able to practically perform basic molecular-biological analyses and interpret the obtained results.
Syllabus
  • General overview of topics:
    1. Isolation of genomic DNA
    2. Characterization of DNA, determination of its concentration and purity by spectrophotometry
    3. Nucleic acid electrophoresis in agarose gel
    4. Genotype determination by PCR, PCR-REA, multiplex PCR, real-time PCR, allele-specific PCR
    5. Consultations, submission of protocols
    6. Final test

    DNA isolation, determination of CCR5 genotype by PCR
    Electrophoresis of CCR5 amplicones, determination of ICAM-1 genotype by PCR-REA
    Restriction cleavage of ICAM-1 amplicons, electrophoresis of NOD2/1007fs genotype determination by multiplex PCR
    Electrophoresis of amplicons of amplicons multiplex PCR, determination of genotype predisposing to celiac disease by allele-specific PCR
    method Electrophoresis of coeliac amplicons, determination of Leiden V allele by real-time PCR
Literature
    recommended literature
  • Altman a kol. Principles of Pharmacogenetics and Pharmacogenomics. 2012. ISBN 9780521885379. info
  • Šmarda J. a kol. Metody molekulární biologie. 2005. ISBN 80-210-3841-1. info
  • Innocenti Federico. Pharmacogenomics - methods and protocols. 2005. ISBN 9781588294401. info
Teaching methods
Laboratory exercises
Assessment methods
The final assessment is a written test (the test consists of 10 questions, 50% of correct answers is needed to pass). During the semester, it is required to prepare protocols from exercises.
Náhradní absolvování
It is not possible to complete the course in a substitute form.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
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