FABL2_16 Biotechnology of Drugs - Practical Classes

Faculty of Pharmacy
Spring 2022
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable of Seminar Groups
FABL2_16/01: each even Friday 8:00–11:20 45-324
Prerequisites
The knowledge of subject in range of lectures.s
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 4/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Learning outcomes
Basic knowledge in methods of pharmaceutical biotechnology
Syllabus
  • 1) Isolation of plant chromosomal DNA
    2) Amplification of selected plant gene by polymerase chain reaction
    3) Purification of PCR products
    4) Isolation of plasmid DNA
    5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
    6) Digestion of vector DNA by restriction endonucleases
    7) Ligation of PCR product into a vector
    8) Transformation of bacterial cells Escherichia coli
    9) PAGE
Literature
    required literature
  • Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
    not specified
  • Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
Teaching methods
LAabs
Assessment methods
Analysis of student work products (technical work)
Language of instruction
English
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is also listed under the following terms Spring 2020, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2022/FABL2_16