B6390p Methods of molecular genetics

Faculty of Science
Spring 2000
Extent and Intensity
2/0/0. 0 credit(s). Type of Completion: -.
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites (in Czech)
B6130 Molecular biology || B4020 Molecular biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Manipulations with nucleic acids, centrifugation. Enzymes in molecular biology - in vitro DNA manipulations. Isolation, identification and characterization of DNA fragments. Electrophoretic and electronmicroscopic analyses of DNA. Restriction analysis of DNA, physical mapping of genomes. DNA sequencing, genomic sequencing. Hybridization techniques, preparation of hybridization probes. Principle of DNA cloning. Prokaryotic cloning vectors. Flow cytmetry. Genomic libraries. Identification of recombinant DNA. Polymerase chain reaction. Transcription and translation in vitro.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2000/B6390p