B6400 Methods of molecular biology

Faculty of Science
Spring 2001
Extent and Intensity
3/0/0. 4 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites (in Czech)
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
Basic techniques in molecular biology. Centrifugation techniques. Electrophoretic and electronmicroscopic methods for DNA analysis. In vitro manipulation with DNA. Enzymes in molecular biology. Isolation, identification and characterization of DNA fragments. Restriction analysis of DNA, physical mapping of genomes. DNA sequencing, genomic sequencing, analysis of DNA sequences. Hybridization techniques, preparation of hybridization probes. Principle of DNA cloning, cloning strategy. Main types of prokaryotic vectors. Gene and genomic libraries. Identification of recombinant DNA, recombinant DNA selection and screening. Expression in E. coli of cloned DNA molecules. Electrophoresis of proteins. Western blotting. Flow cytometry. Polymerase chain reaction. Transcription and translation in vitro.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2000, Spring 2002.
  • Enrolment Statistics (Spring 2001, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2001/B6400