PřF:Bi1050 Biological techniques - Course Information

Bi1050 The Study of Animal and Plant Structure for Biology Teachers

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Milan Baláž, Ph.D. (seminar tutor)
Mgr. Michal Benovics, Ph.D. (seminar tutor)
Mgr. Eva Řehulková, Ph.D. (seminar tutor)

Guaranteed by

Mgr. Eva Řehulková, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Eva Řehulková, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi1050/01: Mon 19. 9. to Sun 18. 12. Thu 7:00–8:50 C13/332, Thu 7:00–8:50 D36/223, M. Baláž, E. Řehulková
Bi1050/02: Mon 19. 9. to Sun 18. 12. Thu 12:00–13:50 C13/332, Thu 12:00–13:50 D36/223, M. Baláž, E. Řehulková

Prerequisites (in Czech)

! Bi2302 Zoological microtechnique && ! Bi1301 Microscopic Techniques && (! NOW ( Bi2302 Zoological microtechnique )) && (! NOW ( Bi1301 Microscopic Techniques ))

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

At the end of this practical course, students should be able
- to use various techniques of light microscopy efficiently
- to prepare various kinds of botanical or zoological mounts
- to take and efectivelly process microphotographs
- to utilize some simple techniques of image analysis
- to conduct simple biological experiment including the statistical treatment of the data
- to utilize basic facts about mitosis, embryogenesis and the other biological processes in their own teaching practice

Syllabus

• Introduction. Light microscope: structure and function. Köhler illumination. Botanical techniques: Microrelief technique for the study of the stomata structure and density. Whole mounts of cleared preparations: development of Angiosperm (dicotyledonous plant) embryo. Squash preparations: cell cycle, mitosis, chromosome counting. Anatomical methods: sectioning (hand sections, fixation, infiltration and embedding, microtome Sections), staining. Documentation - hand drawing, microphotographs and their processing. Simple technique of image analysis - leaf area measurement. Design and statistical treatment of simple biological experiments. Zoological techniques: Native preparations. Vital staining. Whole mounts, sections, squashes, dry mounts. Fixation. Fixatives. Staining. Aqueous media. Non-aqueous mounting media.

Literature

recommended literature
• KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
• PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
• LELLÁKOVÁ, Františka. Zoologická technika. 1. vyd. Praha: Universita Karlova, 1985, 122 s. info
• NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info

Teaching methods

Practical training.

Assessment methods

Whole mounts must be prepared to pass the zoological part of the course. Botanical part of the course: elaboration of all protocols, written test, 75% of correct answers is needed to pass.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi2302 Zoological microtechnique .
  (!Bi1050) && (!NOW(Bi1050))  
• Bi4170 Optical contrast and imaging methods
  Bi1050||Bi2302||Bi1301  

Teacher's information

http://www.sci.muni.cz/kfar/html/biologicka_technika_navody.htm

• Enrolment Statistics (Autumn 2016, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2016/Bi1050