Bi1301 Microscopic Methods for the Study of Plant Structure

Faculty of Science
Autumn 2010
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (seminar tutor)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Timetable of Seminar Groups
Bi1301/01: Mon 12:00–13:50 C13/332, J. Dubová
Bi1301/02: Mon 7:00–8:50 C13/332, J. Dubová
Bi1301/03: Mon 9:00–10:50 C13/332, J. Dubová
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this practical course students should be able to:
describe the historical and present construction of light microscopes;
operate light microscopes in different modes and techniques;
prepare various kinds of botanical microscopic objects;
document these objects by means of microphotography;
process the microphotographs.
Syllabus
  • Introduction to the Methods for the Study of Plant Structure: Light Microscope Development, Structure and Function. Koehler Illumination.
  • Replicas: Study of the Surface Structure by Light Microscope. Stoma. Subsidiary and Neighbour Cells; Stoma Types.
  • Cleared Preparations: Angiosperm Embryo Development.
  • Squash Methods: Cell Cycle, Mitosis, Chromosome Staining.
  • Anatomical Methods: Fixation. Dehydration. Infiltration and Embedding. Paraffin Sections. Sections Staining and Mounting.
  • Vital Preparations: Phase Contrast Microscopy, Nomarski DIC.
  • Pollen: Differential Staining and Pollen Viability
  • Fluorescence Microscopy: Primary and Secondary Fluorescence, Immunofluorescence.
  • Documentation: Measurements. Photomicrography.
Literature
  • HABROVÁ, Věra. Mikroskopická technika. Praha: SPN, 1990. info
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
  • PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
  • NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
  • Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
  • PAZOUREK, Jaroslav. Atlas anatomické stavby rostlin. Praha: Karolinum, 1992. info
  • KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
Teaching methods
Practical laboratory course.
Assessment methods
Protocols of used methods, written test, credit.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2010, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2010/Bi1301