PřF:Bi4020c Essential methods in mol. biol - Course Information

Bi4020c Essential methods in molecular biology

Extent and Intensity

0/1/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.

Timetable

Mon 15:00–15:50 A,01026

Prerequisites

( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology )) && ! Bi4010 Essential molecular biology
Basic knowledge of general genetics, physiology, anorganic chemistry, organic chemistry and biochemistry.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

there are 19 fields of study the course is directly associated with, display

Course objectives

This lecture is conceived as an introduction into molecular biology methods and it is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who are not specialized i molecular biology.

Syllabus

• 1. Stcture of nucleic acids - seminar (primary, secondary and tertiary structure of DNA and RNA, different conformations of DNA and their significance in biological systems).
• 2. Nucleic acids: manipulation, centrifugation, modifications in vitro.
• 3. Electrophoretic analyses of nucleic acids.
• 4. Hybridisation of nucleic acids: probe preparation, labelling.
• 5. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
• 6. Sequence analyses of nucleic acids, genome sequencing.
• 7. DNA cloning (characterization and using of basic types of vectors, cloning strategies, DNA transfer to prokaryotic cells).
• 8. Polymerase chain reaction.
• 9. Basic methods of molecular diagnostics.
• 10. Basic methods of bioinformatics.

Literature

• ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
• ROSYPAL, Stanislav. Úvod do molekulární biologie. Třetí inovované vydání. Brno, 1998, 300 pp. Díl I. Informační makromolekuly. ISBN 80-902562-0-1. URL info

Assessment methods

Examination consists written test.

Language of instruction

Czech

Follow-Up Courses

• Bi6400 Methods of molecular biology
• Bi6405 Methods of Molecular Biology - practice

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• Bi4010c Essential molecular biology - seminar
  !Bi4020c && (NOW(Bi4010) || NOW(Bi4020) || Bi4010 || Bi4020)  
• Bi6336c Biology of germ cells and early embryos - practical course
  Bi4020c  
• Bi7420 Modern methods for genome analysis
  Bi1700 && (Bi4010 || Bi4020) && (Bi4020c || Bi6400)  
• Bi9310 Real-time polymerase chain reaction
  Bi4035 || Bi4020c  
• Bi9310c Real-time polymerase chain reaction – practice
  (Bi4035 || Bi4020c) && NOW(Bi9310)  

• Enrolment Statistics (Spring 2009, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2009/Bi4020c