PřF:Bi6721c Spec.Meth.Microorg.Anal.I.-pr. - Course Information
Bi6721c Special Methods of Microorganisms Analysis I. - practical course
Faculty of ScienceSpring 2022
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. Mgr. Pavel Dvořák, Ph.D. (seminar tutor)
Mgr. Barbora Burýšková (seminar tutor)
Mgr. Barbora Popelářová (seminar tutor) - Guaranteed by
- doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 13:00–18:50 E25/119
- Prerequisites
- NOW ( Bi6721 Spec. Meth. Microorg. Anal. )
Prerequisite for registration of the lab practice is parallel enrollment in the course Special Methods of Microorganisms Analysis (Bi6721). - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10 - fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- The aim of the practical course is to acquaint students with the method of isolation of plasmid and genomic DNA of bacteria using a kit, with the design of oligonucleotide primers and preparation of polymerase chain reaction (end-point and quantitative real-time PCR), with the method of control gel electrophoresis, sequence analysis, and with the purification of the foreign protein from the host bacterium by affinity chromatography.
- Learning outcomes
- At the end of this course, the students should be able to use practically the microbial DNA isolation protocol, the method of polymerase chain reaction, sequence analysis, and the gel electrophoresis method.
- Syllabus
- 1. Preparation of bacterial lysates from cultures of Pseudomonas putida and Escherichia coli.
- 2. Amplification of four specific genes.
- 3. Gel electrophoresis of the amplification, purification of the PCR products.
- 4. Electrophoresis of the purification PCR products, quantification and preparation of the sample for the sending and DNA sequencing.
- 5. In silico analysis of the DNA sequencing.
- 6. Isolation of the genomic DNA - bacterial culture Escherichia coli.
- 7. Estimation of the concentration and purity of the DNA spectrophotometrically.
- 8. Indicative estimation of the concentration and the purity of the DNA spectrofotometrically.
- 9. Polymerase chain reaction by system end-point, gel electrophoresis.
- 10. Real-time PCR with DNA sample from cytomegalovirus.
- 11. Purification of a recombinant protein from Escherichia coli using affinity chromatography
- Literature
- Green, M.R., Sambrook, F. Molecular Cloning. A Laboratory Manual. 4th ed. Cold Spring Harbor Laboratory Press. 2012
Kopecká, J., Bartoš, M. Speciální metody analýzy mikroorganizmů I, návody ke cvičení, 2014
- Green, M.R., Sambrook, F. Molecular Cloning. A Laboratory Manual. 4th ed. Cold Spring Harbor Laboratory Press. 2012
- Teaching methods
- The practice takes place once in 14 days. Students work individually and process their own sample. The results of experiments have to be summarized and evaluated in protocols. The protocols are prepared in accordance (information v the first practice and lecture). In the protocols students describe, compare, analyze, evaluated and discuss own results.
- Assessment methods
- The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course, for the preparation of protocols, and for a brief discussion with the lecture about the prepared protocols.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat ve 2. semestru společně s přednáškou, případně pak ve 4. semestru.
Information on course enrolment limitations: Přednost mají posluchači specializace mikrobiologie.
- Enrolment Statistics (Spring 2022, recent)
- Permalink: https://is.muni.cz/course/sci/spring2022/Bi6721c