Bi7035 Special Virological Techniques

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 4 credit(s). Type of Completion: zk (examination).
Taught in person.
Teacher(s)
prof. RNDr. Daniel Růžek, Ph.D. (lecturer)
RNDr. Luděk Eyer, Ph.D. (lecturer)
RNDr. Jiří Salát, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Daniel Růžek, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. et Mgr. Michal Dvořák
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi5420 General virology
The subject is designated for virology students.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
By the end of the course, the student will be able to understand, explain, and interpret the major current methods and techniques used in virology laboratories and research. Based on the knowledge acquired, he/she will be able to work in a clinical laboratory. He/she will be able to make reasoned decisions based on interpretation of results. The lectures will introduce the main current methods used in virology laboratories. The goal of the course is to learn the basic as well as the advanced experimental methods, techniques and approaches.
Learning outcomes
At the end of the course, the student will be able to understand, explain and interpret the major basic as well as advanced methods used in virology laboratories.
Syllabus
  • Lecture 1-3: Basic introduction to the course, information on course organization. Basics of working in the virology laboratory. Work with infectious material. Classification of virology laboratories. Sterilization and disinfection. The rules of aseptic work.
  • Lecture 4-5: Working with cell cultures. Composition of culture media. Types of cell cultures. Praparation of primary culture. Passaging of the cells. Freezing and thawing of cells. Solving probles with cell cultures.
  • Lecture 5-6: Working with viruses. Determination of virus presence in infectious material. Viral titration. Viral growth curve. Interference test. Hemadsorpce. Virus purification. Keeping frozen viruses. Solving problems with viruses.
  • Lecture 7: Light and electron microscopy in virology. Fluorescence microscopy. Changes of cellular morphology after the infection.
  • Lecture 8: Chicken embryos in virology. Anatomy of chicken embryo. Inoculation of chicken embryo.
  • Lecture 9-10: Work with laboratory animals. Ethics and animal experiments. Breeding laboratory animals. The most commonly used laboratory animals. Anatomy of laboratory animals.
  • Lecture 11-12: serological methods. Methods how to process blood and prepare immune sera. Virus neutralization test. Hemagglutination test, inhibition of hemagglutionation. Other serological methods.
  • Lecture 13: Molecular-biology techniques in virology. Methods of gene engineering in virology.
Literature
  • MISTRÍKOVÁ, Jela. Základné laboratórne techniky používané vo virológii. Univerzita Komenského, 2000. ISBN 978-80-223-1483-1. info
Teaching methods
Theoretical preparation for mastering virological techniques, which are used in practice.
Assessment methods
The final assessment will be in the form of a written test at the end of the semester.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
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