Bi8120 Applied Cell Biology

Faculty of Science
Spring 2011
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
doc. RNDr. Jakub Neradil, Ph.D. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Timetable
Wed 14:00–15:50 B11/235
Prerequisites (in Czech)
Bi3060 Basic genetics && Bi4020 Molecular biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to: understand in detail the specific aspects of the animal cell biology; explain the principles of main methodical approaches in animal cell biology; interpret the main application of the human cell biology in biomedicine (reproductive medicine, cell therapies).
Syllabus
  • 1) INTRODUCTION TO THE ANIMAL CELL BIOLOGY: Organization of the living systems, cellular and non-cellular systems, basic characteristics of the prokaryotic and eukaryotic cells, types of the eukaryotic cells, evolution of the cell.
  • 2) IN VITRO STUDIES OF THE ANIMAL CELLS: In vitro cultivation of the animal cells, tissue cultures and cell cultures, organ cultures, characterization of the diploid and transformed cell lines, practical applications.
  • 3) METHODICAL APPROACHES IN ANIMAL CELL BIOLOGY: Imaging methods, flow cytometry, metabolic assays, study of the living cells, micromanipulation techniques.
  • 4) BIOMEMBRANES: Basic characteristics, compartmentalization - structural and functional aspects, membrane fusion, protoplasts, chimera creation, practical application - hybridoma technology.
  • 5) CYTOSKELETON: Cytoskeletal components, asociated proteins, structural and functional aspects, intracellular transport, motor proteins, cell motility, muscle contraction, nuclear and membrane skeleton.
  • 6) CELL CYCLE AND CELL REPRODUCTION: Phases of the cell cycle, cell cycle checkpoints, regulation of the cell cycle, mitosis, cytokinesis, extracellular control of the cell proliferation.
  • 7) FERTILISATION AND EMBRYOGENESIS: Meiosis a gametogenesis, mechanisms of the fertilisation, embryogenesis, assisted reproduction, reproductive cloning.
  • 8) CELL DIFFERENTIATION: Totipotence, pluripotence, multipotence, stem and progenitor cells, differentiation pathways, growth factors, induced differentiation, failures in the cell differentiation and their consequences.
  • 9) STEM CELLS: Organization and renewal of the tissues, characteristics of the stem cells, adult and embryonic stem cells, cancer stem cells, stem cell lines in the in vitro conditions.
  • 10) CELL THERAPIES: In vitro cultivation of the artifical organ and tissue derivatives, cell therapies, immunotherapies, differentiation therapies, use of the adult and embryonic stem cells, therapeutic cloning.
  • 11) CELL PATHOLOGY: Reaction of the animal cell on the stress condicition, types of the stress factors, physical stress factors (mechanical damage, irradiation, heat shock and cold shock), toxines, specific inhibitors, biological stress factors, practical applications(radiobiology, cryobiology).
  • 12) CELL DEATH: Features of the cell death, cytotoxic and cytostatic effect, programmmed and catastrophic cell death, necrosis and apoptosis, clinical aspects.
Literature
  • NEČAS, Oldřich, Augustin SVOBODA, Milan HEJTMÁNEK, Roman JANISCH, Miroslav ČERVINKA, Karel LENHART and Zdeněk KOLÁŘ. Obecná biologie pro lékařské fakulty (General Biology for Medical Faculties). 3. přepracované, v H+H 1. Jinočany: H+H, 2000, 554 pp. ISBN 80-86022-46-3. info
  • ALBERTS, Bruce. Základy buněčné biologie :úvod do molekulární biologie buňky. 1. vyd. Ústí nad Labem: Espero, 1999, xxvi, 630. ISBN 80-902906-0-4. info
Teaching methods
Lectures.
Assessment methods
written test and oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2011, recent)
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