Bi8670 Plant Biotechnology Principle

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. Ing. Miloš Barták, CSc. (lecturer)
Mgr. Hana Cempírková, Ph.D. (lecturer)
doc. Mgr. Markéta Šámalová, Ph.D. (lecturer)
Guaranteed by
prof. Ing. Miloš Barták, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
SOUHLAS
Students interested in plant biotechnology across levels of plant organization (nanoscale, microbiotechnology, cell, algal biotechnology, secondary metabolites, biotransformation, mutants, in silico photosynthesis, phytoremediation, biotechnology and environment). There are no prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 9 fields of study the course is directly associated with, display
Course objectives
To overview the wide range of topics and complexity of current plant biotechnologies, including prospects for the near future.
Learning outcomes
After completing this course the student will gain theoretical knowledge and will be able to: (1) understand and describe the main groups of current plant biotechnology (2) assess the main controlling variables enabling the application of each biotechnology and their benefits (3) understand and analyse the technical and biological aspects of modern plant biotechnologies, including risk assessment
Syllabus
  • Syllabus 1. Introduction - basic concepts and definitions, historical background of modern plant biotechnology 2. Specific features of biotechnological processes. Advantages, disadvantages and risks of plant biotechnology 3. Algae and cyanobacteria biotechnology biotechnology lines, bioreactors (open, closed type) 4. Bioethanol and oxygen/hydrogen as products of algae/silica biotechnology 5. Plant pigments and secondary metabolites as products of algae/silica biotechnology 6. Biotechnological directions - cultures of plant species (in vitro) 7. Biotechnological trends- enzyme engineering of cells 8. Biotechnological directions- transformation, mutants of A. thaliana, construction of artificial proteins 9. Biotechnological trends- photosynthetic cells in silco 10. Plant biotechnology and the environment- phytoremediation and follow-up technologies 11. Biotechnology and nanotechnology (nanocages, antimicrobial proteins) 12. Current trends: analysis of plant biotechnology case studies. Legal and patent law aspects of biotechnology, ethical issues
Literature
  • ŠÁMALOVÁ, Markéta, C. KIRCHHELLE and I. MOORE. Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species. 2019. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20089. URL info
  • Editing plant genomes with CRISPR/Cas9. Belhaj, K., Chaparro-Garcia, A., Kamoun, S., Patron, N.J. and Nekrasov, V. Current Opinion in Biotechnology 2015, 32:76–84.
  • Applied plant cell biology : cellular tools and approaches for plant biotechnology. Edited by Peter Nick - Zdeněk Opatrný. Berlin: Springer, 2014, xiv, 481. ISBN 9783642417863. info
  • ALBERTS, Bruce, Alexander JOHNSON, Julian LEWIS, David Owen MORGAN, Martin C. RAFF, Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Sixth edition. New York, NY: Garland Science, 2014, xxxiv, 134. ISBN 9780815344322. info
  • Medicinal plant biotechnology. Edited by Rajesh Arora. Cambridge, Mass.: CABI, 2010. xv, 357 p. ISBN 9781845936921
  • Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Zhang, X., Henriques, R., Lin, S.-S., Niu, Q.-W. and Chua, N.-H. NATURE PROTOCOLS 2006, 1:1-6.
  • SAMBROOK, Joseph and David W. RUSSELL. Molecular cloning : a laboratory manual. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2001, 1 sv. ISBN 0879695765. info
Teaching methods
lectures, theory and case studies
Assessment methods
Test and the follow-up oral exam
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
Information about the course of the course Bi8670 Principles of Plant Biotechnology (Spring 2024) Dear Students, You have registered for Bi8670. Which will be taught in the spring semester of 2024 at the Faculty of Science MU Brno. Considering the fact that I am returning from an expedition around the last week of March, the start of the course will be adjusted as follows: In the period of the first to the sixth weeks of the spring semester you will be given 2 lectures, which are guaranteed by Assoc. M. Šámalová and mgr. M.M. Šámalová, M.M. Šámalová and M.M. Hana Cempírková. It is not yet clear how the Bi8670 course will be scheduled (i.e. ´day of the week´ and ´time´. Therefore, please get in touch with both lecturers by e-mail (marketa.samalova@sci.muni.cz, hana.cempirkova@gmail.com ) during the first week of the spring semester and they will let you know the week in which their lectures will be delivered. The rest of the lectures will be given from the 7th to the 12th Week of the semester. I will contact you upon my arrival to discuss the format. There are several options: lectures will be given in the normal, i.e. scheduled, mode. Some of the lectures will then be delivered in agreement with you either at the end of the semester in a block or on alternative dates (weeks 7-12) outside the normal schedule. If you would like to ask any questions, especially about the organization of teaching immediately after my return from Antarctica, please contact my colleague Assoc. J. Hajek (jhajek@sci.muni.cz), who will contact me despite the long distance between the continents and then forward you my answer. In Brno, 19.12.2023 Yours sincerely,
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2016, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (Spring 2025, recent)
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