Bi8670c Plant Biotechnology Principles - Practical Course

Faculty of Science
Spring 2019
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
Mgr. Hana Cempírková, Ph.D. (seminar tutor)
prof. Ing. Miloš Barták, CSc. (seminar tutor)
Mgr. Peter Váczi, Ph.D. (seminar tutor)
Guaranteed by
prof. Ing. Miloš Barták, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Hana Cempírková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
NOW ( Bi8670 Plant Biotechnology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is follow-up of the course in Bi6120 Plant explants, in which students are familiar with various methods of in vitro cultivation. Expands knowledge towards applications of biotechnology for obtaining natural products available for pharmaceutical use ("Pharming"), food and cosmetic purposes, the preparation of edible vaccines, remediation. Part of the course will focus on GMOs, higher plants with resistance to pests, diseases or adverse external influences. From a methodological point of view, mainly an suspension and submerged culture, elicitation, bioconversion, encapsulation and cryopreservation.
Learning outcomes
At the end of this course, students should be able:
- work in sterile conditions used in in vitro plants cultivations;
- prepare media for in vitro plant cultivations;
- use suitable method of in vitro plant cultivation in plant biotechnology (e.g.: callus, suspension cultures);
- suggest and perform selection of transgene plants;
- describe and use some basic molecular techniques (DNA isolation, electrophoresis, PCR);
- describe methods used in preparation of trasgene plants;
- describe and use methods of plant cultivation in bioreactors;
Syllabus
  • 1. Preparation of cultivation media;
  • 2. Work under sterile conditions, sterile seeding on selective medium;
  • 3. Subcultivation of in vitro plants;
  • 4. Transfer of plants in RITA bioreactors;
  • 5. Callus cultures;
  • 6. Initiation and maintenance of suspension cultures;
  • 7. Transformation by Agrobacterium rhizogenes;
  • 8. Organ cultures;
  • 9. Initiation and maintenance of green algea in bioreactors;
  • 10. Plant DNA extraction;
  • 11. SSRs method for testing of somaclonal variability and different plant varieties.
Literature
  • Applied plant cell biology : cellular tools and approaches for plant biotechnology. Edited by Peter Nick - Zdeněk Opatrný. Berlin: Springer. xiv, 481. ISBN 9783642417863. 2014. info
  • Medicinal plant biotechnology. Edited by Rajesh Arora. Cambridge, Mass.: CABI. xv, 357 p. ISBN 9781845936921. 2010. info
  • Plant biotechnology. Edited by R. C. Mishra. Jaipur, India: ABD Publishers. 300 p. ISBN 9788183760232. 2010. info
  • KIRAKOSYAN, Ara and Peter B. KAUFMAN. Recent advances in plant biotechnology. Dordrecht: Springer. xiv, 409. ISBN 9781441901934. 2009. info
  • Medicinal plant biotechnology : from basic research industrial applications. Edited by Oliver Kayser - Wim J. Quax. Weinheim: Wiley-Vch Verlag Gmbh&Co. KGaA. xlii, 265. ISBN 9783527314430. 2007. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons. xii, 303. ISBN 0470021810. 2006. info
  • Potentials of plant biotechnology. Edited by P. B. Bansal. New Delhi, India: Gene-Tech Books. vi, 268 p. ISBN 8189729020. 2006. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press. xxii, 346. ISBN 0199254680. 2003. info
Teaching methods
lab exercises
Assessment methods
test, protocols
Language of instruction
Czech
Further comments (probably available only in Czech)
General note: Předmět je vypisován v jarních semestrech sudých let.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2016, spring 2018, Spring 2023.
  • Enrolment Statistics (Spring 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2019/Bi8670c