C1220 Technologie buněčných kultur

Faculty of Science
Autumn 2023
Extent and Intensity
2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Jan Lochman, Ph.D. (lecturer)
Mgr. Vladimír Rotrekl, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C3181 Biochemistry I || C3580 Biochemistry || C5720 Biochemistry
Basic knowledge of biochemistry, microbiology and molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Different types of microbial, animal and plant cell cultures are introduced. The importance of medium composition and culture parameters will be described. Problems associated with their cultivation are discussed and examples of biotechnological applications are given.
Learning outcomes
At the end of the course the student will be able to explain and describe the principles and methods of microbial, animal and plant cell culture, their main advantages and pitfalls in the context of individual applications in biotechnology.
Syllabus
  • Animal cell cultures (mammalian and insect): general characteristics, sessile and suspension cultures, medium composition, physical parameters, examples of protein production, hybridoma and monoclonal antibody production. Plant cell culture: cell and organ culture, medium composition, bioreactor design, examples of pharmacological metabolite and protein production. Microbial cells: culture methods, the influence of medium composition and physicochemical parameters, a combination of metabolic engineering and culture parameters, examples of metabolite production.
Literature
    recommended literature
  • Aschner Michael. Cell Culture Techniques. Humana Press. 2020. ISBN- 9781493992300
  • Kasper Cornelia. Cell Culture Technology. Springer Nature. 2018. ISBN-9783319748535
Teaching methods
Lecture in the form of PowerPoint slides and demonstration videos.
Assessment methods
Written exam.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/C1220