C7876en Gene technologies - lab.course

Faculty of Science
Spring 2025
Extent and Intensity
0/0/5. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C7875 Gen.tech
Passing of course C7875 Gene technologies.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The laboratory course aims to provide students with practical skills in methods of recombinant DNA technologies, genetic manipulation with bacteria and yeasts, expression of recombinant proteins in E. coli and P. pastoris, bacterial genome sequencing, and transcriptomic and metagenomics analysis.
Learning outcomes
The student will have the following practical skills: 1. Design and implement techniques related to DNA manipulation (cloning, transformation) 2. Design and perform expression of recombinant proteins in the E. coli and P. pastoris yeast expression system 3. To perform and evaluate metagenomics analysis from the environmental sample. 4. To perform and do a basic evaluation of bacterial genome and transcriptome sequencing data. 5. To perform a critical evaluation of acquired data within basic methods of current gene technologies
Syllabus
  • Exercise 1 - Expression of Aspartate-tRNA Ligase Protein in E. coli BL21 (DE3) and its purification by affinity chromatography (IMAC) Exercise 2 - Expression of protein elicitin in P. pastoris GS115 and its subsequent purification by ion-exchange chromatography Exercise 3 - Metagenomics analysis of the environmental sample on the MiniSeq NGS instrument Exercise 4 - Analysis of bacterial transcriptome changes on the MiniSeq NGS instrument Exercise 5 - Bacterial genome sequencing on the MiniSeq NGS instrument Exercise 6 - Evaluation of acquired sequencing data.
Teaching methods
Practical course
Assessment methods
After completing all exercises, an oral presentation presenting measured data and individual problems is done.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2024.
  • Enrolment Statistics (Spring 2025, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2025/C7876en