PřF:C7943 Bioanalytics II - Course Information

C7943 Bioanalytics II - Laboratory medicine

Extent and Intensity

2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

doc. Mgr. Jan Havliš, Dr. (lecturer)

Guaranteed by

doc. Mgr. Jan Havliš, Dr.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites

basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are pre-requisited.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

main objective of the course is to introduce the bioanalytics to its main field - laboratory medicine and diagnostics - to the students.

Learning outcomes

at the end of the course students should be able:
to define relation between bioanalysis and illness diagnosis
to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation
to understand principles of basic methodical approaches and common instrumental techniques.

Syllabus

• 1. – introduction, samples – state specific analyte, sample types and their properties, preanalytical phase
• 2. – classic instrumentation – analysers with liquid and solid reagencies; organisation, integration and miniaturisation of analyses; analytical sets
• 3. – clinical metobolomics – metabolite, metabolome, metabolomics/metabonomics; basic concepts, importance, goals and demands; analytical platforms, data
• 4. – advanced instrumentation – chromatography, electrophoresis, mass spectrometry
• 5. – immunoanalysis – antigen, antibody; methods of Ag-Ab complex detection, immunodiagnostics, immunohistochemistry, immunocytochemistry
• 6. – analysis of proteins – non-specific and specific, protein chips, enzyme analysis, enzyme as analyte and enzyme as analytical agent; role of buffers in bioanalysis
• 7. – colourness – physical phenomenon and its utilisation in bioanalysis; medical microbiology, cultivation, biochemical and proteomic evidence, sensitivity to antibiotics
• 8. – analysis of DNA – genetics and epigenetics in clinical praxis; NA preparation approaches, cytogenetics, identification of known and unknown sequence, methylation analysis
• 9. – quality in clinical analysis – check and control; calibration and reference materials, operative quality control, validation, GLP
• 10. – analytical methods – choice and optimisation; analytical method characteristics, choice of analytical methods
• 11. – analytic result – its presentation; interpretation, uncertainty sources, variability; case study – development of a method for clinical diagnostics
• 12. – selected analytes – inorganic, organic and macromolecular bioanalytes

Literature

• Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis, Mo.: Saunders/Elsevier. xx, 952. ISBN 9780721638652. 2008. info
• Clinical laboratory diagnostics : use and assessment of clinical laboratory results. Edited by Lothar Thomas. 1st ed. Frankfurt am Main: TH-Books. 1527 s. ISBN 3980521540. 1998. info

Teaching methods

the lecture is based on ppt presentation and its explication. presentation itself will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in English covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).

Assessment methods

oral examination; students are required to understand and to be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.

Language of instruction

English

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

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