PřF:C9100 Biosensors - Course Information

C9100 Biosensors

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Thu 16:00–17:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 23 fields of study the course is directly associated with, display

Course objectives

Students will gain overview of biosensors, including electrochemical and optical sensors. The experience on sensors based on affinity interactions, piezoelectric and calorimetric sensors is suitable for characterization of affinity interactions. The obtained knowledge will help to understand principles of the instrumentation available at the department.

Syllabus

• 1. Definition of the biosensor. Historic overview. Characteristics of ideal biosensors. Basic measuring techniques. 2. Electrochemical biosensors, enzyme electrodes. Potentiometric systems and ISFETs. Reference electrodes. 3. Amperometric measurement of oxygen, hydrogen peroxide and NADH, biosensors based on oxidases and dehydrogenases. 4. Electron transfer from enzymes to electrodes using mediators. Composite and organometallic electrodes. 5. Impedimetric and conductometric biosensors. Voltammetric techniques 6. Spectrophotometric, fluorimetric a chemiluminescent sensors, optical fibers. Optical biocatalytic sensors. Bioluminescence. 7. Biosensors for detection of inhibitors. Recycling enzyme systems. Microbial, tissue and receptor-based sensors. 8. Affinity biosensors based on indirect detection of labels. Immunosensors. 9. Hybridization biosensors for detection of nucleic acids and oligonucleotide sequences. 10. Direct optical affinity sensors. Evanescent wave and surface plasmon resonance utilized for monitoring of bioaffinity interactions in real time. Integrated optical systems, interferometers and related techniques. 11. Immobilization of biomolecules for construction of biosensors. Membrane techniques. Electropolymerization. 12. Activation of sensing surfaces. Covalent immobilization of biomolecules. 13. Miniaturization and mass production of biosensors. Screen-printing, litography and mass production of biosensors. Biosensors in integrated analytical microsystems. Biochips. 14. Commercial biosensors. Perspectives of biosensors, applications in clinical medicine, food production, environmental monitoring, military application.

Literature

• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry : life at the molecular level. 3rd ed. Hoboken, N.J.: John Wiley & Sons. xxx, 1099. ISBN 9780470129302. 2008. URL info

Teaching methods

lecture course

Assessment methods

Participation at lectures required, oral examination - random selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Teacher's information

http://www.sci.muni.cz/~skladal/

• Enrolment Statistics (Autumn 2013, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2013/C9100