F9410A Bioelectrochemistry

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
prof. RNDr. Vladimír Vetterl, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Vetterl, DrSc.
Timetable
Thu 7:00–8:50 BFU
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • Biophysics (programme PřF, N-FY)
  • Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika)
  • Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika)
Course objectives
The main objectives of the course is to give students information on electrochemical properties (faradayic and tensammetric processes at the electrodes) of nucleic acids and proteins and their monomeric components - purine and pyrimidine bases, nucleoside, nucletides, amino acids.
Syllabus
  • 1. Electrocapillary curves, potential of electrocapillary maximum. 2. Electrode double layer. 3. Electrochemical methods. 3.1. Polarography. 3.2. Impedance measurements, electrochemical impedance spectroscopy. 3.3. Measurement of electrocapillary curves. 4. Adsorption 4.1. Effect of adsorption on electrocapillary curves 4.2. Adsorption isotherms. 5. Two-dimensional condensation, Avrami equation. 6. Electrochemical properties of nucleic acids. 6.1. Faradayic properties, reduction, oxydation. 6.2. Tensammetric properties. 6.3. Electric field effect on the conformation of adsorbed nucleic acids.
Literature
  • J.Kůta and E.Paleček: J. Electroanal,. Chem. 536: 19-35, 2002
  • Brabec, V.Kleinwächter and V.Vetterl: Structure, chemical reactivity and electromagnetic properties of nucleic acids., Bioelectrochemistry: Principles and Practice 1997, p.1-104.
  • S. Hasoň, S.-P. Simonaho, R. Silvennoinen and V. Vetterl: On the adsorption and kinetics of phase transients of adenosine at the different carbon electrodes modified with a mercury layer. Electrochim. Acta 48: 651-668, 2003.
  • V.Brabec, V.Vetterl and O.Vrána:Bioelectrochemistry: Principles and Practice Vol.3 1996, p.287-359
  • S. Hasoň and V. Vetterl: Application of carbon electrodes modified with mercury layer of a different thickness for studies of the adsorption and kinetics of phase transients of cytidine, J. Electroanal,. Chem. 536: 19-35, 2002
  • Hasoň, J. Dvořák, F. Jelen and V. Vetterl: Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes, Crit. Rev. Anal. Chem. 32: 167 - 169, 2002.
  • E. Paleček, M. Fojta, F. Jelen and V. Vetterl: Electrochemical analysis of nucleic acids., in Encyclopedia of Electrochemistry Vol.9. Bioelectrochemistry Chapter 13 2002, p.365 - 430.
Assessment methods
1 written test, colloqium
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012.
  • Enrolment Statistics (Autumn 2008, recent)
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