PřF:S2012 Mendel’s laws in plant biology - Informace o předmětu

S2012 Applying Mendel’s laws of genetics to plant biology in the lab

Rozsah

1/2/0. 3 kr. (plus ukončení). Doporučované ukončení: zk. Jiná možná ukončení: k.
Vyučováno kontaktně

Vyučující

Helene Robert Boisivon, Ph.D. (přednášející)
Samia Belaidi, M.Sc. (pomocník)
doc. Mgr. Jan Havliš, Dr. (pomocník)

Garance

Helene Robert Boisivon, Ph.D.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Kontaktní osoba: Helene Robert Boisivon, Ph.D.
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta

Rozvrh

Po 9:00–9:50 E26/222, Po 10:00–11:50 E26/222

Předpoklady

The target audience is BSc and MSc students. During the course, all necessary information will be provided. However, a basic knowledge of genetics is welcome.

Omezení zápisu do předmětu

Předmět je otevřen studentům libovolného oboru.
Předmět si smí zapsat nejvýše 10 stud.
Momentální stav registrace a zápisu: zapsáno: 5/10, pouze zareg.: 0/10, pouze zareg. s předností (mateřské obory): 0/10

Cíle předmětu

This course will use various laboratory techniques to test the segregation law of genetics as established by G. Mendel, the correlation between genotype and phenotype, and the effects of plant hormones for plant development. The lectures are accompanied by practical work.

Výstupy z učení

Students should be able to understand the analysis of segregation for a single genetic character, and the link between genotype and phenotype. Students will learn basic lab techniques. Students will be initiated to the role of plant hormones in plant development.

Osnova

• Using Arabidopsis mutants the students will observe and calculate the frequencies of the mutant’s phenotypes, and correlate it with the genotype of individual seedlings. This part of the practical uses laboratory techniques such as work in sterile conditions, sow seeds for in vitro culture, monitoring seedling growth and analysis of the seedling phenotypes by stereomicroscope.
• In a second part of the course, the student will investigate the effects of the plant hormone auxin on root growth (few concentrations tested) and on reporter lines for the hormone signalling pathway. Activation or repression of the signalling pathways will be tested by colorimetric staining. Results will be collected and conclusion will be written and discussed. This part of the practical includes tissue culture as in the first part (including preparation of the growth medium), result analysis (root growth measurements), colorimetric staining and microscopy.

Výukové metody

Lectures, exercises and practical work. Sharing information and documents will be done through IS interactive syllabus.

Metody hodnocení

Test and practical report.
Evaluation of the course is based on short exercises and practical report.

Vyučovací jazyk

Angličtina

Informace učitele

Planned schedule

Mon. 2/10 9.00, room: E26/222

Theory part 1 (Role of auxin for plant development, Auxin signalling and auxin transport, pin1 and monopteros mutants)

Preparative lecture Practical 1

Fri. 6/10 9.00, E26 labs

Practical part 1

Mon. 9/10 9.00, E26 labs

Practical part 1

Fri. 13/10 9.00, room: E26/222

Theory part 2 (Mendel’s law of genetics, Molecular genetics in Arabidopsis thaliana)

Mon. 16/10, E26 labs (full day)

Practical part 1

Mon. 23/10, E26 labs (full day)

Practical part 1

Mon. 30/10, 9.00, E26/222

Theory part 3 (Root development, Effects of auxin for root growth)

Preparative lecture Practical 2

Practical part 1

Mon. 13/11 9.00, E26 labs

Practical part 2

Wed. 15/11, E26 labs

Practical part 2

Mon. 20/11 9.00, E26 labs

Practical part 2

Wed. 22/11 9.00, E26 labs

Practical part 2

Fri. 24/11 9.00, E26 labs

Practical part 2

Mon. 27/11 9.00, E26 labs

Practical part 2

Fri. 1/12 9.00, room: E26/222

Practical part 2

Mon. 8/1

Report handling

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