BLBC0211c Biochemistry - practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. David Galuška (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Thu 17. 2. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 24. 2. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 3. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 10. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 17. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 24. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 31. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 7. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 14. 4. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 21. 4. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 28. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 5. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 12. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 19. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 26. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212
Prerequisites (in Czech)
BLLC0111s Medical Chemistry - seminar && BLLC0111c Medical Chemistry - practice && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the work with biological material and with the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids and with the basic methods of interpretation of laboratory results.
Learning outcomes
After completing the course, the student will be:
- acquainted with the basic methodological principles and procedures used in the clinical laboratory in determining the concentration of analytes and enzyme activity in body fluids.
- able to follow the principles of working with biological material.
- able to orientate in basic laboratory tasks.
- able to interpret the result of laboratory examination.
Syllabus
  • Handling biological material. Anticoagulants and their use for blood plasma separation. Communicating results of tests in clinical biochemistry: request forms and laboratory reports.
  • Analysis of the absorption spectra of haemoglobin, oxyhaemoglobin and carbonylhaemoglobin. Determination of haemoglobin in blood. Urine test for haemoglobin.
  • Determination of total bilirubin in serum. Estimation of bilirubin, urobilinogens and urobilins in urine.
  • Determination of total serum proteins by biuret reagent, determination of albumin. Precipitation tests for urine proteins. Detection of proteinuria by diagnostic strips.
  • Determination of alanine aminotransferase activity in serum. Determination of alkaline phosphatase activity in serum.
  • The enzymatic assay of glucose in blood and urine. Oral glucose tolerance test. Estimation of glycaemia by diagnostic strips. Detection of glucose in urine.
  • Determination of total cholesterol in serum. Principles of HDL-cholesterol determination and calculation of the LDL-cholesterol concentration. Chromatography of serum lipids.
  • The solubility of uric acid. Murexide test for uric acid. Detection of uric acid in renal calculi. Determination of uric acid in serum and urine.
  • Determination of urea in serum and urine. Approximate assessment of the nitrogen balance.
  • Appearance and volume of urine, specific gravity and osmolality of urine. Urine pH measurement. Determination of creatinine in blood, serum and urine. Creatinine clearance calculation, calculation of tubular reabsorption and fractional excretion of water.
  • Tests in routine urinalysis employing chemical principle: determination of pathological components, multipurpose diagnostic strips in urinalysis.
  • Simplified chemical analysis of renal calculi. Practice in urinalysis, seeking for pathological constituents in urine.
  • Determination of gastric acid output. Tubeless gastric analyses. Detection of lactic acid.
Literature
    required literature
  • Návody ke cvičením dostupné v IS MUNI.
Teaching methods
Lab exercises
Assessment methods
Credit. The condition for granting the credit is:
  • completion of all exercises,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI,
  • successful completion of the credit test (at least 70% of points),
  • return of the borrowed key from the locker.
    The detailed conditions are posted in Information System (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu BLBC0211p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    https://is.muni.cz/auth/el/1411/jaro2022/BLBC0211c/
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.

    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required for the exercise.

    Consultations are possible after a personal arrangement with the teacher.

    The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2023, spring 2024.
    • Enrolment Statistics (spring 2022, recent)
    • Permalink: https://is.muni.cz/course/med/spring2022/BLBC0211c