LF:BLKHM0422c Histopath. tech. II - Course Information

BLKHM0422c Histopathological techniques II

Extent and Intensity

0/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

MUDr. Jitka Kyclová (lecturer)
PhDr. Květoslava Matulová (seminar tutor)
Michaela Gregorovičová (assistant)
Jarmila Jelínková (assistant)

Guaranteed by

PhDr. Květoslava Matulová
Department of Pathology – Joint workplaces with the University Hospital Brno - workplaces of the Bohunice and Mater. Hospital – Faculty of Medicine
Contact Person: MUDr. Jitka Kyclová
Supplier department: Department of Pathology – Joint workplaces with the University Hospital Brno - workplaces of the Bohunice and Mater. Hospital – Faculty of Medicine

Timetable

Fri 22. 3. 8:00–12:10 P01014, Fri 12. 4. 8:00–12:10 P01014, Fri 10. 5. 8:00–12:10 P01014, Fri 31. 5. 8:00–12:10 P01014

Prerequisites

BLKHM0321c Histopath. Tech. I
The student have to have completed the subject Histopathological examination methods I.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Laboratory Assistant (programme LF, B-SZ)

Course objectives

This subject is devoted to application of histologic methods in practical pathomorphologic diagnostics (bioptic and necroptic). Student will get to know the practical operation in histopathologic laboratory and obtain skills in demonstration of constituent tissue components by means of histologic, histochemical and immunohistochemical techniques as well as some procedures of molecular biology. Within scope of this course, student will learn recent methods of histopathologic diagnostics of tumors and other pathomorphologic units.

Learning outcomes

After finishing the course the student will be able to: - prepare formalin-fixed and paraffin-embedded tissue blocks - cut the tissue slices and place them on to glass microscope slides and perform routine hematoxylin-eosin staining and special staining - process cytological material - process intraoperative biopsy

Syllabus

• Collecting of material for histologic examination. Fixation – physical and chemical fixatives.
• Dehydration, clearing, embedding into paraffin and celloidin, cutting on sliding and rotary microtomes, cryostat.
• Staining of histologic sections: types of histologic staining dyes and staining methods. Synoptic staining: hematoxylin – eosine (HE), Weigert-van Gieson, trichromes, AZAN, Mallory staining. Impregnation, evidence of inorganic substances, reticular fibers, pigments.
• Structure and work of Department of pathology. Laboratory segments within pathology. Equipment of reception office, dispatch note.
• Types of bioptic collection - biopsy during operation, trepanobiopsy, transport of bioptic material, microexcision, puncture.
• Adjustment of tissue block, processing machines, registration of samples, time relations for expedition of bioptic findings.
• Autopsy, structure and working content of autoptic segment of pathology. Didactic segment of pathologic department.
• Cytodiagnostic, preparation of cytologic smears, cytoblocks, cytospin. Cytologic diagnostics of uterine cervix. Cytologic diagnostics in pneumology, cytologic screening.
• Histochemistry, basic types of reactions. Collecting of material, fixation, freezing, cryostat sections for histochemical reactions (enzymes, lipids, glycides).
• Histochemical demonstration of enzymes, basic types of reactions. Evidence of enzymes at malabsorption syndrome and in myopathology.
• Classification and histochemistry of lipids, basic staining, extraction methods to distinguish polar and apolar lipids. Application – steatosis.
• Classification and histochemistry of glycides, PAS reaction, staining with alcian blue, application on gastronitestinal tract biopsies. Histochemical demonstration of amyloid, copper and iron.
• Fundamentals of immunohistochemical reactions, mono- and polyclonal antibodies.
• Intermedial filaments (cytokeratins, vimentin, desmin, GFAP, neurofilaments), signification for specification of tissue types and their tumorous derivatives. Steps in immunohistochemical diagnostic of tumors.
• CD antigens and antibodies, application in hematology and in diagnostic of malignant lymphomas.
• Molecular pathologic methods in diagnostic. Principles of fluorescence in situ hybridization (FISH).
• Principles of PCR. Advantage and disadvantage of this technique. Differences between immunohistochemical analysis and immunobloting.

Literature

recommended literature
• JIRKOVSKÁ, Marie. Histologická technika : pro studenty lékařství a zdravotnické techniky. 1. vyd. Praha: Galén, 2006, 80 s. ISBN 8072622633. info

not specified
• BALKO, Jan, Zbyněk TONAR and Ivan VARGA. Memorix histologie. Edited by Radovan Hudák. 2. doplněné vydání. Praha: Triton, 2017, 555 stran. ISBN 9788075532497. info
• Theory and practice of histological techniques. Edited by John D. Bancroft - Marilyn Gamble. 5th ed. Edinburgh: Churchill Livingstone, 2003, xiii, 796. ISBN 0443064350. info
• VACEK, Zdeněk. Histologie a histologická technika. 1. vyd. Brno: Institut pro další vzdělávání pracovníků ve zdravotnictví, 1995, 184 s. ISBN 80-7013-202-7. info

Teaching methods

Demonstration and practical implementation of representative histopathologic methods in laboratories of Department of pathology.

Assessment methods

The exam is written, students respond 42questions selected by lot out of following areas: basic histologic methods and electron microscopy, methods of histochemistry and immunohistochemistry, methods of cytology, methods of molecular pathology.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Všechny formy kontaktní výuky předmětu vyjma přednášek jsou povinné.
Information on the extent and intensity of the course: 10.

• Enrolment Statistics (spring 2019, recent)
  
• Permalink: https://is.muni.cz/course/med/spring2019/BLKHM0422c