BLLC0111c Medical Chemistry - practice

Faculty of Medicine
autumn 2020
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
Mgr. Martina Drabinová (assistant)
Martina Hanousková (assistant)
Miriam Minaříková (assistant)
Mgr. Lucie Novotná (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Thu 13:00–15:30 A16/216, Thu 13:00–15:30 A16/211
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are to become oriented in the environment of the chemical laboratory and its equipment, to acquire simple laboratory procedures and techniques, to practice common separation procedures and become familiar with handling standard laboratory machines. Students will learn to handle laboratory data into the lab reports, to solve the problems and derive conclusions.
Learning outcomes
Students
- will be familiar with regulations for safe handling, use, and disposal of chemicals
- will be able to handle with basal laboratory equipment and follow proper procedures
- will pipette and weigh according to the principles of good laboratory practice
- will understand the relationship between the composition of different solutions and their pH value
- will be able to describe the general composition and role of the buffers, and prepare a buffer
- to describe the essence of the formation of insoluble and poorly soluble compounds, to identify factors that can influence the insolubility of compounds
- will be oriented in relations between a structure od bioorganic molecules and their reactivity
- will explain principles of chromatographic methods, their applications and utilization
- will be able to apply photometric methods for determination of solution concentration
Syllabus
  • Handling chemicals. Laws on the handling of chemical substances and preparations.
  • Introduction, organizational guidelines. Safety and health protection at work in the laboratory, principles of first aid.
  • Measuring containers / aids, plastics. Heating, cooling, weighing. Cylinders.
  • Measuring small volumes of liquids, calibrating pipettes, expressing accuracy and precision.
  • Preparation of solutions.
  • Filtration, dialysis, ultrafiltration, centrifugation. Concentration of samples.
  • Measurement and calculation of pH solutions of acids, bases and salts, hydrolysis, pH-meters.
  • Neutralization volumetric analysis. Titration neutralization curves.
  • Buffer solutions and methods for their preparation, determination of buffering capacity.
  • Ionic reactions, ions in drinking water, ion-selective electrodes, solubility of substances.
  • Chromatography - basic types and techniques. Water demineralization.
  • Spectrophotometry.
  • Reactions of organic compounds.
  • Solubility of proteins, methods of their quantification.
Literature
    required literature
  • Podklady pro praktická cvičení v IS.
Teaching methods
The course is realized in laboratories. Students are doing simple experiments according to the instructions in the textbook. On the base of results, they prepare the lab-reports.
Assessment methods
The condition for giving credit is:
  • the completion of all exercises, incl. submission of all lab reports,
  • credit from the subject ZC011,
  • and achievement at least 70% success from tests writing during the semester. If the student does not achieve at least 70% success, he writes a credit test. Credit is then given upon successful completion of the credit test (achievement of at least 46% success rate). Students have the opportunity to repeat the credit test only once. Depending on epidemiological situation, assessment method may be altered. The current conditions will be posted in IS (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    Information on completion of the course: ukončení předmětu je podmíněno úspěšným ukončením předmětu ZC011.
    The course is taught annually.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2021, autumn 2022, autumn 2023.
    • Enrolment Statistics (autumn 2020, recent)
    • Permalink: https://is.muni.cz/course/med/autumn2020/BLLC0111c