BLSM041c Statistical methods and procedures in laboratory practice

Faculty of Medicine
Spring 2016
Extent and Intensity
0/1. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Petr Breinek (lecturer)
Mgr. Jana Flášarová (lecturer)
RNDr. Alena Mikušková (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
doc. MUDr. Milan Dastych, CSc., MBA (seminar tutor)
prof. RNDr. Zdeněk Glatz, CSc. (assistant)
Michaela Gregorovičová (assistant)
doc. RNDr. Oldřich Janiczek, CSc. (assistant)
doc. RNDr. Petr Zbořil, CSc. (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Prerequisites
copletion of subjects Clinical Biochemistry II; MBKB051p, MBKB051c
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This course will teach the student the application of statistical procedures and methods for a routine workflow in a laboratory of clinical biochemistry. He/she will be capable of utilizing and evaluating the statistical procedures of internal control of quality in order to discern the systematic or random errors of measurement. The student will be introduced to and will be able to use the systems of quality control in automatic biochemistry analyzers, laboratory information systems and external systems of quality control and will be competent to work with external quality control systems (SEKK). He/She will learn about biochemical tests utilization, will be able to calculate the total uncertainty of test results and use them for clinical interpretation. The student will learn how to verify the external subcontractor’s reference intervals, will be able to assess the diagnostic specificity and sensitivity of various tests and to determine the optimal cut-off value via ROC curve and related parameters. He/She will be acquainted with the requirements of Czech Society for Clinical Biochemistry (ČSKB) for method validation and verification and will be able to perform them.
Learning outcomes
He/she will be capable of utilizing and evaluating the statistical procedures of internal control of quality in order to discern the systematic or random errors of measurement. The student will be introduced to and will be able to use the systems of quality control in automatic biochemistry analyzers, laboratory information systems and external systems of quality control and will be competent to work with external quality control systems (SEKK). He/She will learn about biochemical tests utilization, will be able to calculate the total uncertainty of test results and use them for clinical interpretation. The student will learn how to verify the external subcontractor’s reference intervals, will be able to assess the diagnostic specificity and sensitivity of various tests and to determine the optimal cut-off value via ROC curve and related parameters. He/She will be acquainted with the requirements of Czech Society for Clinical Biochemistry (ČSKB) for method validation and verification and will be able to perform them.
Syllabus
  • Analytical method errors • Validation & Verification of analytic methods • System of internal quality control • Delta check • Westgard rules • System of external control of quality • Uncertainties • Specificity & Sensitivity • Reference ranges • Cut-off value • ROC curve • Method comparison
Literature
  • Učební texty k jednotlivým tématům budou poskytnuty v rámci přednášek a cvičení
Teaching methods
The course will take the form of lectures with interactive elements and practical demonstrations. The topics will be reinforced by application of the statistical procedures in the computer lecture room as a part of practical exercises. The practice will be applied to the real laboratory data from Dep. of Clinical Biochemistry University Hospital Brno.
Assessment methods
The course is finished by written exam.
Language of instruction
Czech
The course is also listed under the following terms Spring 2012, Spring 2013, Spring 2014, Spring 2015.
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