VLIM051c Immunology - practice

Faculty of Medicine
autumn 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
MUDr. Katarína Dítětová (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/02: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/03: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/04: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/05: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/06: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/07: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Tue 10:00–11:40 ÚKIA N01021
VLIM051c/10: Tue 10:00–11:40 ÚKIA N01021
VLIM051c/11: Thu 10:00–11:40 ÚKIA N01021
VLIM051c/12: Thu 10:00–11:40 ÚKIA N01021
VLIM051c/13: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/14: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/15: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/16: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/17: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/18: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/19: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/20: Fri 10:00–11:40 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry II - lecture || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022.

VLIM051c Immunology - practice

Faculty of Medicine
autumn 2022
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
MUDr. Katarína Dítětová (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/02: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/03: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/04: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/06: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/07: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/08: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/09: Thu 10:00–11:40 ÚKIA N01021
VLIM051c/10: Thu 10:00–11:40 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/14: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/15: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/16: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/17: Tue 10:30–12:10 ÚKIA N01021
VLIM051c/18: Tue 10:30–12:10 ÚKIA N01021
VLIM051c/19: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/20: Wed 8:00–9:40 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry II - lecture || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
autumn 2021
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
MUDr. Katarína Dítětová (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Mon 13. 9. to Fri 17. 12. Tue 8:00–9:40 ÚKIA N01021
VLIM051c/02: Mon 13. 9. to Fri 17. 12. Tue 8:00–9:40 ÚKIA N01021
VLIM051c/03: Mon 13. 9. to Fri 17. 12. Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Mon 13. 9. to Fri 17. 12. Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 13. 9. to Fri 17. 12. Mon 10:00–11:40 ÚKIA N01021
VLIM051c/06: Mon 13. 9. to Fri 17. 12. Mon 10:00–11:40 ÚKIA N01021
VLIM051c/07: Mon 13. 9. to Fri 17. 12. Mon 8:00–9:40 ÚKIA N01021
VLIM051c/08: Mon 13. 9. to Fri 17. 12. Mon 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 13. 9. to Fri 17. 12. Fri 10:00–11:40 ÚKIA N01021
VLIM051c/10: Mon 13. 9. to Fri 17. 12. Fri 10:00–11:40 ÚKIA N01021
VLIM051c/11: Mon 13. 9. to Fri 17. 12. Fri 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 13. 9. to Fri 17. 12. Fri 8:00–9:40 ÚKIA N01021
VLIM051c/13: Mon 13. 9. to Fri 17. 12. Tue 10:00–11:40 ÚKIA N01021
VLIM051c/14: Mon 13. 9. to Fri 17. 12. Tue 10:00–11:40 ÚKIA N01021
VLIM051c/15: Mon 13. 9. to Fri 17. 12. Wed 10:00–11:40 ÚKIA N01021
VLIM051c/16: Mon 13. 9. to Fri 17. 12. Wed 10:00–11:40 ÚKIA N01021
VLIM051c/17: Mon 13. 9. to Fri 17. 12. Thu 8:00–9:40 ÚKIA N01021
VLIM051c/18: Mon 13. 9. to Fri 17. 12. Thu 8:00–9:40 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry I -lect. || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • CHAPEL, Helen, Mansel HAENEY, Siraj A. MISBAH and Neil SNOWDEN. Základy klinické imunologie : 6. vydání. Translated by Vojtěch Thon. Kroměříž: Triton, 2018, xiv, 343. ISBN 9788075533968. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
autumn 2020
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
MUDr. Katarína Dítětová (seminar tutor)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/02: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/06: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/07: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/08: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/09: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/10: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/11: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/12: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/16: Wed 10:00–11:40 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry II - lecture || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • CHAPEL, Helen, Mansel HAENEY, Siraj A. MISBAH and Neil SNOWDEN. Základy klinické imunologie : 6. vydání. Translated by Vojtěch Thon. Kroměříž: Triton, 2018, xiv, 343. ISBN 9788075533968. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
autumn 2019
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/02: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/06: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/07: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/08: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/09: Tue 10:00–11:40 ÚKIA N01021
VLIM051c/10: Tue 10:00–11:40 ÚKIA N01021
VLIM051c/11: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/12: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/14: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/15: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/16: Wed 10:00–11:40 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry I -lect. || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • CHAPEL, Helen, Mansel HAENEY, Siraj A. MISBAH and Neil SNOWDEN. Základy klinické imunologie : 6. vydání. Translated by Vojtěch Thon. Kroměříž: Triton, 2018, xiv, 343. ISBN 9788075533968. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
autumn 2018
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/02: Wed 10:00–11:40 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/06: Mon 10:00–11:40 ÚKIA N01021
VLIM051c/07: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/08: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/09: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/10: Fri 10:00–11:40 ÚKIA N01021
VLIM051c/11: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/12: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:00–11:40 ÚKIA N01021
VLIM051c/14: Tue 10:00–11:40 ÚKIA N01021
VLIM051c/15: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/16: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/17: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/18: Tue 8:00–9:40 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry I -lect. || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • CHAPEL, Helen, Mansel HAENEY, Siraj A. MISBAH and Neil SNOWDEN. Základy klinické imunologie : 6. vydání. Translated by Vojtěch Thon. Kroměříž: Triton, 2018, xiv, 343. ISBN 9788075533968. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2017
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Mon 25. 9. to Fri 22. 12. Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Mon 25. 9. to Fri 22. 12. Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Mon 25. 9. to Fri 22. 12. Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Mon 25. 9. to Fri 22. 12. Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 25. 9. to Fri 22. 12. Mon 10:10–11:50 ÚKIA N01021
VLIM051c/06: Mon 25. 9. to Fri 22. 12. Mon 10:10–11:50 ÚKIA N01021
VLIM051c/07: Mon 25. 9. to Fri 22. 12. Mon 8:00–9:40 ÚKIA N01021
VLIM051c/08: Mon 25. 9. to Fri 22. 12. Mon 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 25. 9. to Fri 22. 12. Fri 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 25. 9. to Fri 22. 12. Fri 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 25. 9. to Fri 22. 12. Fri 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 25. 9. to Fri 22. 12. Fri 8:00–9:40 ÚKIA N01021
VLIM051c/13: Mon 25. 9. to Fri 22. 12. Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Mon 25. 9. to Fri 22. 12. Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Mon 25. 9. to Fri 22. 12. Thu 8:00–9:40 ÚKIA N01021
VLIM051c/16: Mon 25. 9. to Fri 22. 12. Thu 8:00–9:40 ÚKIA N01021
VLIM051c/17: Mon 25. 9. to Fri 22. 12. Thu 10:10–11:50 ÚKIA N01021
VLIM051c/18: Mon 25. 9. to Fri 22. 12. Thu 10:10–11:50 ÚKIA N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry I -lect. || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017, 297 stran. ISBN 9788075532503. info
  • CHAPEL, Helen, Mansel HAENEY, Siraj A. MISBAH and Neil SNOWDEN. Základy klinické imunologie : 6. vydání. Translated by Vojtěch Thon. Kroměříž: Triton, 2018, xiv, 343. ISBN 9788075533968. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practice

Faculty of Medicine
Autumn 2016
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Mon 26. 9. to Fri 6. 1. Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Mon 26. 9. to Fri 6. 1. Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Mon 26. 9. to Fri 6. 1. Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Mon 26. 9. to Fri 6. 1. Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Mon 26. 9. to Fri 6. 1. Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Mon 26. 9. to Fri 6. 1. Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Mon 26. 9. to Fri 6. 1. Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Mon 26. 9. to Fri 6. 1. Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 26. 9. to Fri 6. 1. Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 26. 9. to Fri 6. 1. Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 26. 9. to Fri 6. 1. Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 26. 9. to Fri 6. 1. Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Mon 26. 9. to Fri 6. 1. Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Mon 26. 9. to Fri 6. 1. Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Mon 26. 9. to Fri 6. 1. Thu 8:00–9:40 ÚKIA N01021
VLIM051c/16: Mon 26. 9. to Fri 6. 1. Thu 8:00–9:40 ÚKIA N01021
VLIM051c/17: Mon 26. 9. to Fri 6. 1. Thu 10:10–11:50 ÚKIA N01021
VLIM051c/18: Mon 26. 9. to Fri 6. 1. Thu 10:10–11:50 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
VLIM051c/34: No timetable has been entered into IS.
VLIM051c/35: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med. Chemistry - lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří. Základy vyšetření v klinické imunologii. 2., přepracované vydání. Brno: Masarykova univerzita, 2015, 71 stran. ISBN 9788021078536. info
    not specified
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 5. vydání. Praha: Triton, 2013, 330 stran. ISBN 9788073877132. info
  • ABBAS, Abul K., Andrew H. LICHTMAN and Shiv PILLAI. Basic immunology : functions and disorders of the immune system. Fifth edition. St. Louis: Elsevier, 2016, x, 335. ISBN 9780323390828. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Presence in 90% of seminars with active participation in discussions is required. The knowledge is continuously checked by oral questions. The student must participate in ppreparation od crientific articles presentation or presentaion of case reports.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practice

Faculty of Medicine
Autumn 2015
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021, J. Litzman
VLIM051c/15: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/16: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021, V. Thon
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
VLIM051c/34: No timetable has been entered into IS.
VLIM051c/35: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med. Chemistry - lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2011, 53 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 4th ed. Praha: Triton, 2009, 306 pp. ISBN 978-80-7387-280-9. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Presence in 90% of seminars with active participation in discussions is required. The knowledge is continuously checked by oral questions. The student must participate in ppreparation od crientific articles presentation or presentaion of case reports.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2014
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021, J. Litzman
VLIM051c/15: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/16: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021, V. Thon
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
VLIM051c/34: No timetable has been entered into IS.
VLIM051c/35: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I - lect. && ( VSLC011p Med. Chemistry - lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II -lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2011, 53 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 4th ed. Praha: Triton, 2009, 306 pp. ISBN 978-80-7387-280-9. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Presence in 90% of seminars with active participation in discussions is required. The knowledge is continuously checked by oral questions. The student must participate in ppreparation od crientific articles presentation or presentaion of case reports.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2013
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/19: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/20: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
VLIM051c/34: No timetable has been entered into IS.
VLIM051c/35: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med. Chemistry - lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics -lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2011, 53 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 4th ed. Praha: Triton, 2009, 306 pp. ISBN 978-80-7387-280-9. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Presence in 90% of seminars with active participation in discussions is required. The knowledge is continuously checked by oral questions. The student must participate in ppreparation od crientific articles presentation or presentaion of case reports.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2012
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/19: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/20: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med. Chem. lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will employ in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. Also immunological problems associated with transplantation and malignancies will demonstrated.A selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentation, discussion and critical assessment is also included.
Assessment methods
To get the credit, presence in 90% of seminars with active participation in discussions is required. The knowledge is. continuously checked by oral questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2011
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/04: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/08: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/19: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/20: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will employ in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. Also immunological problems associated with transplantation and malignancies will demonstrated.A selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentation, discussion and critical assessment is also included.
Assessment methods
To get the credit, presence in 90% of seminars with active participation in discussions is required. The knowledge is. continuously checked by oral questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2010
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/04: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/08: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/19: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/20: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will employ in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. Also immunological problems associated with transplantation and malignancies will demonstrated.A selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentation, discussion and critical assessment is also included.
Assessment methods
To get the credit, presence in 90% of seminars with active participation in discussions is required. The knowledge is. continuously checked by oral questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2009
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/02: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/03: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/04: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/05: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/06: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/07: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/08: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/18: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/19: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/20: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will employ in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. Also immunological problems associated with transplantation and malignancies will demonstrated.A selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentation, discussion and critical assessment is also included.
Assessment methods
To get the credit, presence in 90% of seminars with active participation in discussions is required. The knowledge is. continuously checked by oral questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2008
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Fri 10:40–12:30 ÚKIA N01021
VLIM051c/02: Fri 10:40–12:30 ÚKIA N01021
VLIM051c/03: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/04: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/05: Mon 10:40–12:30 ÚKIA N01021
VLIM051c/06: Mon 10:40–12:30 ÚKIA N01021
VLIM051c/07: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/08: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/09: Tue 10:40–12:30 ÚKIA N01021
VLIM051c/10: Tue 10:40–12:30 ÚKIA N01021
VLIM051c/11: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/12: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/13: Thu 10:40–12:30 ÚKIA N01021
VLIM051c/14: Thu 10:40–12:30 ÚKIA N01021
VLIM051c/15: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/16: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/17: Wed 10:40–12:30 ÚKIA N01021
VLIM051c/18: Wed 10:40–12:30 ÚKIA N01021
VLIM051c/19: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/20: Wed 7:30–9:10 ÚKIA N01021
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -seminar || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment). In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed. Also immunological problems associated with transplantation and malignancies will be dealt with. After finishing this course the student will understand the pathogenesis of immunopathological diseases and know the clinical manifestation and the treatment approaches to the most severe disturbances of the immune system.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions. Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology. Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation. Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells. B lymphocytes and immunoglobulins. . Biological properties of antibodies. Primary and secondary immunodeficiency disorders. HIV disease. Active and passive immunisation. Immunoglobulin therapy. Immunostimulation. Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies. Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated. Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock. Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Assessment methods
The course is given in a form of seminars. To get the credit, presence in 90% of seminars is required.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2007
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/02: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/03: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/04: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/05: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/06: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/07: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/08: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/18: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/19: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/20: Thu 7:30–9:10 ÚKIA N01021
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2006
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Timetable of Seminar Groups
VLIM051c/01: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/02: Wed 9:30–11:10 ÚKIA N01021
VLIM051c/03: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/04: Wed 7:30–9:10 ÚKIA N01021
VLIM051c/05: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/06: Fri 9:30–11:10 ÚKIA N01021
VLIM051c/07: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/08: Fri 7:30–9:10 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/15: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/16: Tue 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/18: Thu 9:30–11:10 ÚKIA N01021
VLIM051c/19: Thu 7:30–9:10 ÚKIA N01021
VLIM051c/20: Thu 7:30–9:10 ÚKIA N01021
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical ethics
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused to clinical and laboratory aspects of immunology in medicine. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment). In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed. Also immunological problems associated with transplantation and malignancies will be dealt with. After finishing this course the student will understand the pathogenesis of immunopathological diseases and know the clinical manifestation and the treatment approaches to the most severe disturbances of the immune system.
Syllabus
  • Clinical immunology laboratory Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions. Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology. Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation. Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells. B lymphocytes and immunoglobulins. . Biological properties of antibodies. Primary and secondary immunodeficiency disorders. HIV disease. Active and passive immunisation. Immunoglobulin therapy. Immunostimulation. Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies. Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated. Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock. Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1st ed. Brno: Masarykova univerzita, 2007, 59 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
Assessment methods
Givng of the credit is conditioned by active attendance of 90% of the pracical lessons.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2005
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && ( VSAJ0222 English 1 II -p || VSNJ0222 German 1 II -p || VSRJ0222 Russian 1 II -p || VSSJ0222 Spanish 1 II -p || VSFJ0222 French 1 II -p || VSCJ0484 Czech for Foreigners IV -p ) && VSET021 Medical ethics
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2004
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && ( VSBF011p Biophysics || VLBF011p Biophysics ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && ( VSAJ0222 English 1 II -p || VSNJ0222 German 1 II -p || VSRJ0222 Russian 1 II -p || VSSJ0222 Spanish 1 II -p || VSFJ0222 French 1 II -p || VSCJ0484 Czech for Foreigners IV -p ) && VSET021 Medical ethics
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2003
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Prerequisites (in Czech)
VSBC0221p Biochemistry I -lect. && ( VSLC011p Med.Chem.- lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II - lecture && VSBF011p Biophysics && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && ( VSAJ0222 English 1 II -p || VSNJ0222 German 1 II -p || VSRJ0222 Russian 1 II -p || VSSJ0222 Spanish 1 II -p || VSFJ0222 French 1 II -p || VSCJ0484 Czech for Foreigners IV -p ) && VSET021 Medical ethics
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2002
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2001
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
MUDr. Ivo Nentwich, Ph.D. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2000, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2000
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
MUDr. Ivo Nentwich, Ph.D. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.
  • Enrolment Statistics (recent)